Isolation of cDNAs encoding novel transcription coactivators p52 and p75 reveals an alternate regulatory mechanism of transcriptional activation
Isolation of cDNAs encoding novel transcription coactivators p52 and p75 reveals an alternate regulatory mechanism of transcriptional activation
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DOI：
10.1093/emboj/17.22.6723
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发表时间：
1998-11-16
期刊：
影响因子：
11.4
通讯作者：
Roeder, RG
中科院分区：
文献类型：
--
作者：
Ge, H;Si, YZ;Roeder, RG
在含有RNA聚合酶II和一般起始因子的人类无细胞系统中，转录激活需要一种或多种额外的共激活因子的作用。在这里，我们报告的cDNA编码两种新的人类转录辅激活因子（p52和p75），是来自一个单一的基因的选择性剪接产物和共享一个区域的325个残基的分离，但表现出不同的辅激活因子的属性。p52和p75都显示出与VP16激活结构域和一般转录机制的几个组分的强相互作用。p52，像前面描述的PC4，是一种有效的广泛特异性共激活因子，而p75对大多数激活结构域的活性较低。这些结果表明，p52是一个通用的转录辅激活因子，介导上游序列特异性激活因子和一般的转录装置之间的功能相互作用，可能通过一种新的机制。
Transcriptional activation in human cell-free systems containing RNA polymerase II and general initiation factors requires the action of one or more additional coactivators. Here, we report the isolation of cDNAs encoding two novel human transcriptional coactivators (p52 and p75) that are derived from alternatively spliced products of a single gene and share a region of 325 residues, but show distinct coactivator properties. p52 and p75 both show strong interactions with the VP16 activation domain and several components of the general transcriptional machinery. p52, like the previously described PC4, is a potent broad-specificity coactivator, whereas p75 is less active for most activation domains. These results suggest that p52 is a general transcriptional coactivator that mediates functional interactions between upstream sequence-specific activators and the general transcription apparatus, possibly through a novel mechanism.