SAW1 is required for SDSA double-strand break repair in S. cerevisiae
SAW1 is required for SDSA double-strand break repair in S. cerevisiae
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DOI：
10.1016/j.bbrc.2014.02.048
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发表时间：
2014-03-14
影响因子：
3.1
通讯作者：
Fischhaber, Paula L.
中科院分区：
文献类型：
--
作者：
Diamante, Graciel;Claire Phan;Fischhaber, Paula L.
SAW 1基因编码Saw 1基因，是酿酒酵母单链退火DNA双链断裂修复所必需的。Saw 1与Rad 1和Rad 52物理结合并募集Rad 1-Rad 10内切核酸酶。在此，我们通过荧光显微镜显示，SAW 1是同样需要的招聘Rad 10的合成依赖链退火（SDSA）的网站，并与网站的SDSA修复在时间上与Rad 10重叠的方式。SDSA中Saw 1-CFP/Rad 10-YFP/DSB-RFP共定位灶的诱导幅度在S和G2期细胞中比在M期更显著，与SDSA的已知机制一致。我们观察到一个相当大的分数的病灶，其中Rad 10被定位到修复网站没有Saw 1，但很少DSB网站包含Saw 1没有Rad 10。这些数据与Saw 1将Rad 1-Rad 10招募到SDSA位点的模型一致，甚至可能作为蛋白质-蛋白质复合物结合，但在Rad 1-Rad 10之前离开修复位点。(C)2014爱思唯尔公司All rights reserved.
SAW1, coding for Saw1, is required for single-strand annealing (SSA) DNA double-strand break (DSB) repair in Saccharomycescerevisiae. Saw1 physically associates with Rad1 and Rad52 and recruits the Rad1-Rad10 endonuclease. Herein we show by fluorescence microscopy that SAW1 is similarly required for recruitment of Rad10 to sites of Synthesis-Dependent Strand Annealing (SDSA) and associates with sites of SDSA repair in a manner temporally overlapped with Rad10. The magnitude of induction of colocalized Saw1-CFP/Rad10-YFP/DSB-RFP foci in SDSA is more dramatic in S and G2 phase cells than in M phase, consistent with the known mechanism of SDSA. We observed a substantial fraction of foci in which Rad10 was localized to the repair site without Saw1, but few DSB sites that contained Saw1 without Rad10. Together these data are consistent with a model in which Saw1 recruits Rad1-Rad10 to SDSA sites, possibly even binding as a protein-protein complex, but departs the repair site in advance of Rad1-Rad10. (C) 2014 Elsevier Inc. All rights reserved.