A HOLISTIC APPROACH TO TAXONOMIC EVALUATION OF TWO CLOSELY RELATED ENDANGERED FRESHWATER MUSSEL SPECIES, THE OYSTER MUSSEL EPIOBLASMA CAPSAEFORMIS AND TAN RIFFLESHELL EPIOBLASMA FLORENTINA WALKERI (BIVALVIA: UNIONIDAE)
A HOLISTIC APPROACH TO TAXONOMIC EVALUATION OF TWO CLOSELY RELATED ENDANGERED FRESHWATER MUSSEL SPECIES, THE OYSTER MUSSEL EPIOBLASMA CAPSAEFORMIS AND TAN RIFFLESHELL EPIOBLASMA FLORENTINA WALKERI (BIVALVIA: UNIONIDAE)
复制标题
对两种密切相关的濒危淡水贻贝物种——牡蛎贻贝 Epioblasma capsaeformis 和 Tan RIFFLESHELL EPIOBLASMA FLORENTINA WALKERI（双壳类：Unionidae）的分类学评估的整体方法
DOI：
10.1093/mollus/eyl004
复制
发表时间：
2006
影响因子：
1.2
通讯作者：
E. Hallerman
中科院分区：
文献类型：
--
作者：
Jess W. Jones;R. Neves;S. Ahlstedt;E. Hallerman
Epioblasma属的物种有专门的生活史要求，是世界上最濒危的淡水贻贝属（蚌科）。对牡蛎E. capsaeformis和tanriffleshellE.进行florentina walkeri sensu late是为了评估分类学的有效性，并解决恢复规划的保护问题。这些贻贝物种表现出明显的表型变异，但很难用DNA序列来表征遗传学特征。单系谱系，一致的物种之间的表型变异，只有经过广泛的分析相结合的线粒体（1396 bp的16 S，细胞色素b，ND 1）和核（515 bp的ITS-1）DNA序列。相比之下，在10个高变DNA微卫星位点的变异分析显示中度到高度分歧的人口的基础上FST和ESTA值，范围从0.12至0.39和0.15至0.71，分别。不同种间的鱼宿主特异性存在数量上的差异，其中E. capsaeformis对羽镖鲈的抑制作用显著大于（P，0.05）; F.扇尾镖鲈的Walkeri指数显著高于扇尾镖鲈（P0.05）。钩介类外套膜的平均大小在241 ~ 272 mm之间，种间和居群间差异极显著（P <0.001）。capsaeformis stricto为光滑的蓝白色，而E. F.沃克里长了脓包，呈棕色，有棕褐色的斑点。根据大量的分子、形态和生活史资料，对该属的种群进行了初步的研究。capsaeformis作为一个独立种，并将E. F.美国弗吉尼亚州克林奇河上游印第安溪的沃克里被认为是一个独特的亚种。
Species in the genus Epioblasma have specialized life history requirements and represent the most endangered genus of freshwater mussels (Unionidae) in the world. A genetic characterization of extant populations of the oyster mussel E. capsaeformis and tan riffleshell E. florentina walkeri sensu late was conducted to assess taxonomic validity and to resolve conservation issues for recovery planning. These mussel species exhibit pronounced phenotypic variation, but were difficult to characterize phylogenetically using DNA sequences. Monophyletic lineages, congruent with phenotypic variation among species, were obtained only after extensive analysis of combined mitochondrial (1396 bp of 16S, cytochrome-b, and ND1) and nuclear (515 bp of ITS-1) DNA sequences. In contrast, analysis of variation at 10 hypervariable DNA microsatellite loci showed moderately to highly diverged populations based on FST and RST values, which ranged from 0.12 to 0.39 and 0.15 to 0.71, respectively. Quantitative variation between species was observed in fish-host specificity, with transformation success of glochidia of E. capsaeformis significantly greater ( P , 0.05) on greenside darter Etheostoma blennioides, and that of E. f. walkeri significantly greater ( P , 0.05) on fantail darter Etheostoma flabellare. Lengths of glochidia differed significantly ( P , 0.001) among species and populations, with mean sizes ranging from 241 to 272 mm. The texture and colour of the mantle-pad of E. capsaeformis sensu stricto is smooth and bluish-white, whereas that of E. f. walkeri is pustuled and brown, with tan mottling. Based on extensive molecular, morphological and life history data, the population of E. capsaeformis from the Duck River, Tennessee, USA is proposed as a separate species, and the population of E. f. walkeri from Indian Creek, upper Clinch River, Virginia, USA is proposed as a distinct subspecies.
DOI：
10.1073/pnas.92.11.4957
发表时间：
1995-05-23
影响因子：
11.1
作者：
SWANSON, WJ;VACQUIER, VD
通讯作者：
VACQUIER, VD