Molecular evidence of Bartonella spp. in questing adult Ixodes pacificus ticks in California
Molecular evidence of Bartonella spp. in questing adult Ixodes pacificus ticks in California
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DOI：
10.1128/jcm.39.4.1221-1226.2001
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发表时间：
2001-04-01
影响因子：
9.4
通讯作者：
Tietze, N
中科院分区：
文献类型：
--
作者：
Chang, CC;Chomel, BB;Tietze, N
蜱是美国许多人畜共患疾病的媒介，包括莱姆病、人单核细胞和粒细胞埃立克体病以及落基山斑疹热。大多数已知的巴尔通体属物种是节肢动物传播的。因此，重要的是要确定一些巴尔通体属，这是新兴的病原体，是否可以携带或传播的蜱。在这项研究中，成年硬蜱pacificus蜱收集了标记植被在三个地点在圣克拉拉县，加利福尼亚州。PCR-限制性片段长度多态性和273 bp的gltA基因的部分测序用于巴尔通体的鉴定。29（19.2%）的151个单独测试的蜱为巴尔通体PCR阳性。雄蜱比雌蜱更容易感染巴尔通体（26比12%，P = 0.05）。在Baird Ranch采集的9只蜱虫中无一对巴尔通体呈PCR阳性。然而，7（50%）的14蜱从红蕨牧场和22（17%）的128蜱从风山开放空间保护区感染巴尔通体。在这些感染的蜱中，分子分析显示了多种巴尔通体菌株，这些菌株与牛巴尔通体菌株和几种已知的人类致病性巴尔通体种属和亚种密切相关：汉赛巴尔通体B。金塔纳河washoensis和B.温氏亚种伯克霍菲。这些发现表明，I.太平洋蜱可能在巴尔通体在动物和人类之间的传播中起重要作用。
Ticks are the vectors of many zoonotic diseases in the United States, including Lyme disease, human monocytic and granulocytic ehrlichioses, and Rocky Mountain spotted fever. Most known Bartonella species are arthropod borne. Therefore, it is important to determine if some Bartonella species, which are emerging pathogens, could he carried or transmitted by ticks. In this study, adult Ixodes pacificus ticks were collected by flagging vegetation in three sites in Santa Clara County, Calif. PCR-restriction fragment length polymorphism and partial sequencing of 273 bp of the gltA gene were applied for Bartonella identification. Twenty-nine (19.2%) of 151 individually tested ticks were PCR positive for Bartonella. Male ticks were more likely to he infected with Bartonella than female ticks (26 versus 12%, P = 0.05). None of the nine ticks collected at Baird Ranch was PCR positive for Bartonella. However, 7 (50%) of 14 ticks from Red Fern Ranch and 22 (17%) of 128 ticks from the Windy Hill Open Space Reserve were infected with Bartonella. In these infected ticks, molecular analysis showed a variety of Bartonella strains, which were closely related to a cattle Bartonella strain and to several known human-pathogenic Bartonella species and subspecies: Bartonella henselae B. quintana, R. washoensis, and B. vinsonii subsp. berkhoffii. These findings indicate that I. pacificus ticks may play an important role in Bartonella transmission among animals and humans.