Preparation of hexagonal GeO2 particles with particle size and crystallinity controlled by peptides, silk and silk-peptide chimeras.
Preparation of hexagonal GeO2 particles with particle size and crystallinity controlled by peptides, silk and silk-peptide chimeras.
复制标题
制备六方GeO2颗粒，其粒径和结晶度受肽、丝和丝肽嵌合体控制。
DOI：
10.1039/c4dt01974a
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Boix E
中科院分区：
文献类型：
--
作者：
Boix E
我们证明了使用丝基蛋白质来控制颗粒/微晶尺寸在GeO 2形成过程中，使用仿生方法在circumneutral pH值和环境温度。在不同的蚕丝蛋白质存在下，从四乙醇锗（TEOG）制备多晶GeO 2：桑蚕丝（天然丝）和两种嵌合蛋白质，通过在不同的肽负载量（丝-Ge 28 10%和丝-Ge 28 50%）下将锗结合肽（Ge 28：HATGTHGLSLSH）与桑蚕丝通过化学偶联制备。将基于丝的蛋白质的矿化活性与作为对照系统的肽Ge 28的矿化活性进行比较。GeO 2矿化研究在水中和柠檬酸/bis-tris丙烷缓冲液在pH 6。对所获得的材料的形态、粒度、结晶度、水和有机物含量进行了分析，以研究添加的生物分子和矿化环境对材料性能的影响。在蚕丝添加剂的存在下，可以得到由六方晶系氧化锗和最多约100种金属元素组成的立方体形杂化材料。获得5wt%有机物含量。立方颗粒的尺寸范围为0.4 - 1.4 μm，由35-106 nm范围内的晶畴组成，取决于所使用的添加剂和合成条件。矿物中的有机物质似乎不会影响晶胞尺寸。水中的丝和嵌合蛋白可能通过有效的离子通道机制促进材料形成和晶体生长，但需要进一步研究来确定丝的存在对成核和生长阶段的影响程度。与空白相比，单独的氧化锗结合肽对反应速率、产率或材料性质没有任何显著影响。有趣的是，测试的丝嵌合体中的肽含量并不影响矿化。缓冲剂的存在抑制了矿物的缩合速率和产率。基于丝的生物分子的使用允许控制混合材料的微晶/颗粒尺寸，从而为生物启发方法应用于功能性氧化锗基器件和材料的合成开辟了机会。
We demonstrate the use of silk based proteins to control the particle/crystallite size during GeO2 formation, using a bio-mimetic approach at circumneutral pH and ambient temperature. Multicrystalline GeO2 was prepared from germanium tetraethoxide (TEOG) in the presence of different silk-based proteins: Bombyx mori silk (native silk) and two chimeric proteins prepared by linking a germania binding peptide (Ge28: HATGTHGLSLSH) with Bombyx mori silk via chemical coupling at different peptide loadings (silk-Ge28 10% and silk-Ge28 50%). The mineralisation activity of the silk-based proteins was compared with that of peptide Ge28 as a control system. GeO2 mineralisation was investigated in water and in citric acid/bis-tris propane buffer at pH 6. Morphology, particle size, crystallinity, water and organic content of the materials obtained were analysed to study the effect of added biomolecules and mineralisation environment on material properties. In the presence of silk additives well-defined cube-shape hybrid materials composed of hexagonal germania and up to ca. 5 wt% organic content were obtained. The cubic particles ranged from 0.4 to 1.4 μm in size and were composed of crystalline domains in the range 35–106 nm depending on the additive used and synthesis conditions. The organic material incorporated in the mineral did not appear to affect the unit cell dimensions. The silk and chimeric proteins in water promote material formation and crystal growth, possibly via an effective ion-channelling mechanism, however further studies are needed to assert to what extent the presence of the silk impacts on nucleation and growth stages. The germania binding peptide alone did not have any significant effect on reaction rate, yield or the material's properties compared to the blank. Interestingly, the peptide content in the silk chimeras tested did not affect mineralisation. The presence of buffer inhibited mineral condensation rate and yield. The use of silk-based biomolecules allows control of crystallite/particle size of hybrid materials opening up opportunities for bio-inspired approaches to be applied for the synthesis of functional germania based devices and materials.