Insulinotropic action of bombesin-like peptides mediated by gastrin-releasing peptide receptors in steers.
Insulinotropic action of bombesin-like peptides mediated by gastrin-releasing peptide receptors in steers.
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牛体内胃泌素释放肽受体介导的铃蟾肽样肽的促胰岛素作用。
DOI：
10.2527/jas.2015-9495
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发表时间：
2016
影响因子：
3.3
通讯作者：
H. Kuwayama
中科院分区：
文献类型：
--
作者：
H. Zhao;G. Yao;S. Yannaing;S. Thanthan;H. Kuwayama
本研究的特点受体介导的促胰岛素作用的蛙皮素样肽（BLP）在反刍动物。随机给8头荷斯坦牛静脉注射人工合成的牛胃泌素释放肽（GRP; 0.9 nmol/kg BW），神经介肽B（NMB; 0.9 nmol/kg BW）或神经介肽C（NMC; 0.9 nmol/kg BW），各自单独或与GRP受体拮抗剂N-乙酰基-GRP-OCHCH组合（N-GRP-EE; 22.5 nmol/kg BW）或GH促分泌素受体1a型（GHS-R1 a）拮抗剂[D-Lys]-GHRP-6（21.5 nmol/kg BW）。相对于注射时间，在-10、0（注射前）、5、10、15、20、30、45、60、75和90 min采集血样。分析血浆中注射的肽、胰岛素和葡萄糖的水平。结果显示，注射NMC或GRP后5 min胰岛素水平出现峰值。在2个阶段中观察到血浆葡萄糖;与注射媒介物相比，在施用NMC或GRP后显著升高，随后显著下降（< 0.05）。在相同摩尔浓度下，GRP对胰岛素和葡萄糖的作用强于NMC（< 0.05）。N-GRP-EE可完全阻断NMC引起的胰岛素和葡萄糖的变化，而[D-Lys]-GHRP-6则不能阻断这些变化。单独施用NMB或N-GRP-EE在任何采样时间点期间都没有改变胰岛素或葡萄糖的循环水平（> 0.05）。这些结果表明BLP的促胰岛素作用是由GRP受体介导的，而不是通过ghrelin/GHS-R1 a途径，BLP可能参与调节反刍动物的葡萄糖稳态。
The present study characterizes the receptor that mediates the insulinotropic action of bombesin-like peptides (BLP) in ruminants. Eight Holstein steers were randomly and intravenously injected with synthetic bovine gastrin-releasing peptide (GRP; 0.9 nmol/kg BW), neuromedin B (NMB; 0.9 nmol/kg BW), or neuromedin C (NMC; 0.9 nmol/kg BW), each alone or combined with the antagonist of GRP receptors N-acetyl-GRP-OCHCH (N-GRP-EE; 22.5 nmol/kg BW) or the antagonist of GH secretagogue receptor type 1a (GHS-R1a) [D-Lys]-GHRP-6 (21.5 nmol/kg BW). Blood samples were collected at -10, 0 (just before injection), 5, 10, 15, 20, 30, 45, 60, 75, and 90 min relative to injection time. Levels of injected peptides, insulin, and glucose in plasma were analyzed. Results showed that the peak of insulin levels was seen at 5 min after injection of NMC or GRP. Plasma glucose was observed in 2 phases; a significant rise followed a remarkable fall after NMC or GRP administration compared with injection of the vehicle ( < 0.05). On a same molar basis, effects of GRP on insulin and glucose were more potent than those of NMC ( < 0.05). The NMC-induced changes of insulin and glucose were completely blocked by N-GRP-EE, but [D-Lys]-GHRP-6 did not block any of these changes. Administration of NMB or N-GRP-EE alone did not change the circulating levels of insulin or glucose during any of the sampling time points ( > 0.05). These results indicated that the insulinotropic action of BLP is mediated by GRP receptors but not through a ghrelin/GHS-R1a pathway and that BLP may be involved in the regulation of glucose homeostasis in ruminants.
影响因子：
7.7
作者：
Y. Date;M. Nakazato;S. Hashiguchi;K. Dezaki;M. S. Mondal;H. Hosoda;M. Kojima;K. Kangawa;T. Arima
通讯作者：
Y. Date;M. Nakazato;S. Hashiguchi;K. Dezaki;M. S. Mondal;H. Hosoda;M. Kojima;K. Kangawa;T. Arima