Amplitude distribution of calcium sparks in confocal images:: Theory and studies with an automatic detection method
Amplitude distribution of calcium sparks in confocal images:: Theory and studies with an automatic detection method
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DOI：
10.1016/s0006-3495(99)77229-2
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发表时间：
1999-02-01
影响因子：
3.4
通讯作者：
Stern, MD
中科院分区：
文献类型：
--
作者：
Cheng, H;Song, LS;Stern, MD
钙火花幅度分布的确定对于了解横纹肌中基本钙释放事件的性质是至关重要的。在目前的研究中，我们表明，在一般的理论基础上，在共焦线扫描图像中观察到的钙火花应该具有非模式、单调递减的幅度分布，无论潜在的事件是否是刻板印象的。为了验证这一预测，我们开发、实现并验证了一种自动计算机算法，用于客观检测和测量原始图像数据中的钙火花。当算法的灵敏度和可靠性设置适当时，我们在骨骼肌细胞和心肌细胞中观察到高度左偏或单调下降的幅度分布，证实了理论预测。因此，先前报道的人眼检测到的火花的模式或高斯分布必然是对小幅度事件的主观检测偏差的结果。此外，我们还讨论了可能观察到模式分布的情况。
Determination of the calcium spark amplitude distribution is of critical importance for understanding the nature of elementary calcium release events in striated muscle. In the present study we show, on general theoretical grounds, that calcium sparks, as observed in confocal line scan images, should have a nonmodal, monotonic decreasing amplitude distribution, regardless of whether the underlying events are stereotyped. To test this prediction we developed, implemented, and verified an automated computer algorithm for objective detection and measurement of calcium sparks in raw image data. When the sensitivity and reliability of the algorithm were set appropriately, we observed highly left-skewed or monotonic decreasing amplitude distributions in skeletal muscle cells and cardiomyocytes, confirming the theoretical predictions. The previously reported modal or Gaussian distributions of sparks detected by eye must therefore be the result of subjective detection bias against small amplitude events. In addition, we discuss possible situations when a modal distribution might be observed.