Gas chromatography mass spectrometry analysis of the cuticular hydrocarbons from parasitic wasps of the genus Muscidifurax
Gas chromatography mass spectrometry analysis of the cuticular hydrocarbons from parasitic wasps of the genus Muscidifurax
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DOI：
10.1016/s1044-0305(97)00288-2
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发表时间：
1998-04-01
影响因子：
3.2
通讯作者：
Geden, CJ
中科院分区：
文献类型：
--
作者：
Bernier, UR;Carlson, DA;Geden, CJ
通过传统的分类技术很难识别寄生膜翅目。角质层碳氢化合物（CHC）的检查为化学分类学分化提供了基础，这可能导致信息素的发现，并且可以成为检查菌落物种交叉污染的一种手段。所检查的寄生蜂为 Muscidifurax raptor、M. zaraptor、M, uniraptor 和群居形式的 M, raptorellus。通过检查 CHC 的气相色谱图可以清楚地区分蝇属内的物种以及性别。通过质谱法鉴定烷烃显示该属成员不常见的二甲基烷烃和三甲基烷烃。与文献报道的昆虫中发现的甲基支化角质层碳氢化合物相比，这些昆虫的甲基支化角质层碳氢化合物很少，但在这些昆虫上却大量存在。此外，在这些物种的雄性和雌性角质层表面存在的长链烷烃 (C-21-C-39) 中观察到性别二态性。雌性的角质层烃往往具有位于二甲基、三甲基和四甲基烷烃碳主链外部的甲基支链，而雄性往往具有位于烃主链内部的二甲基和三甲基烷烃。提出了在这些寄生蜂上鉴定的新型和罕见的甲基支链化合物的质谱。
Parasitic Hymenoptera can be difficult to identify by conventional taxonomic techniques. Examination of the cuticular hydrocarbons (CHCs) provides a basis for chemotaxonomic differentiation, which may lead to the discovery of pheromones, and can be a means of examining colonies for species cross-contamination. The parasitic wasps examined were Muscidifurax raptor, M. zaraptor, M, uniraptor, and the gregarious form of M, raptorellus. Species within the genus Muscidifurax, as well as the sex, can clearly be differentiated by examining the gas chromatograms of the CHCs. Identification of the alkanes by mass spectrometry shows uncommon dimethylalkanes and trimethylalkanes for members of the genus. The methyl branched cuticular hydrocarbons of these insects are rare compared to those found on insects reported in the literature, but are present in significant amounts on these insects. Additionally, sexual dimorphism is observed in long chain alkanes (C-21-C-39) present on male and female cuticular surfaces for these species. Females tend to have cuticular hydrocarbons with methyl branches located externally on the carbon backbone chain for dimethyl-, trimethyl-, and tetramethylalkanes, whereas males tend to have dimethyl-and trimethylalkanes located internally on the hydrocarbon backbone chains. Mass spectra of novel and rare methyl branched compounds identified on these parasitoids are presented.