Impact of Hematopoietic Progenitor Cell Count as an Indicator for Optimal Timing of Peripheral Stem Cell Harvest in Clinical Practice
Impact of Hematopoietic Progenitor Cell Count as an Indicator for Optimal Timing of Peripheral Stem Cell Harvest in Clinical Practice
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DOI：
10.3960/jslrt.56.150
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发表时间：
2017-03-01
影响因子：
1.5
通讯作者：
Kakuta, Shinichiro
中科院分区：
文献类型：
--
作者：
Tanaka, Hiroaki;Ishii, Akihiro;Kakuta, Shinichiro
为了优化CD34+细胞收集，适当的外周血干细胞收获（PBSCH）起始时间是至关重要的。具有未成熟骨髓信息通道的自动细胞分析仪提供造血祖细胞（HPC）计数，这是CD34+细胞的替代标记物，可以在几分钟内获得，而不需要单克隆抗体。利用收获当天早晨获得的HPC计数，可以做出对PBSCH发起的最终决定。在此，我们评估了HPC计数作为临床实践中PBSCH最佳时机指标的影响，超过9年。来自72例的118个细胞株在PBSC产量中有一定数量的CD34+细胞/kg。外周血中HPC计数与CD34+细胞计数有相关性（R = 0.563, p < 0.001），白细胞计数与CD34+细胞计数无相关性（R = 0.0418, p = 0.65）。我们定义在单次分离中，> 2.0 × 10(6)/kg的CD34+细胞为良好的动员。多因素分析表明，HPC计数> 21/ μ L，成髓细胞计数> 12/ μ L，以及PBSCH年龄< 50岁与良好的动员分别独立相关（p = 0.001, p < 0.001和p = 0.005）。我们的研究结果表明，HPC计数是PBSCH最佳时机的一个很好的指标。
For optimizing CD34+ cell collection, appropriately timing peripheral blood stem cell harvest (PBSCH) initiation is crucial. Automatic cell analyzers with the immature myeloid information channel provide hematopoietic progenitor cell (HPC) count, a surrogate marker of CD34+ cells, which can be obtained within a few minutes without requiring monoclonal antibodies. The final decision on PBSCH initiation can be made using the HPC count obtained on the morning of the harvest day. Herein, we evaluated the impact of the HPC count as an indicator for the optimal timing of PBSCH in clinical practice over 9 years. One hundred and eighteen aphereses from 72 cases had a definite number of CD34+ cells/kg in the PBSC yield. A correlation was found between the HPC count in the PB and the CD34+ cell count (R = 0.563, p < 0.001), whereas no correlation existed between the white blood cell and CD34+ cell counts (R = 0.0418, p = 0.65). We defined > 2.0 x 10(6)/kg of CD34+ cells in a single apheresis as good mobilization. Multivariate analysis demonstrated that an HPC count of > 21/mu L, myeloblast count of > 12/mu L, and age at PBSCH of < 50 years were independently associated with good mobilization (p = 0.001, p < 0.001, and p = 0.005, respectively).Our findings suggest that the HPC count is a good indicator for the optimal timing of PBSCH.