Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial
Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial
复制标题
天南青蛙皮肤分泌物中的宿主防御肽：青蛙除抗菌之外的其他生存策略的证据。
DOI：
10.1016/j.biochi.2011.09.017
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发表时间：
2012-03-01
期刊：
影响因子：
3.9
通讯作者：
Yu, Haining
中科院分区：
文献类型：
--
作者：
He, Weiyu;Feng, Feifei;Yu, Haining
臭蛙属是所有研究过的两栖动物中抗菌肽含量最丰富、种类最多的一种。在我们的前期工作中，从田南臭蛙皮肤中获得了46个编码22种抗菌肽前体的cDNA序列。本文报道了从O.天南它们的氨基酸序列与cDNA序列完全一致，分别命名为Odorranain-C7 HSa、Brevinin-1-OT 2和Odorranain-G-OT。此外，我们选择分析了22个AMP中结构最多样化的四个序列，这些序列与所有报道的AMP都有显著差异。经结构鉴定，其中3个分别命名为pleurain-E-OT、odorranain-G-OT、odorranain-A-OT，属于AMP家族。第四个具有独特的AILTTLANWARKFLa和C-末端酰胺化的14-mer序列，代表了一类新的两栖动物AMP的原型，因此命名为tiannanensin。序列和结构的这种广泛多样性与臭蛙属的其他物种一致。对合成的4种抗菌肽进行了抗菌、抗氧化、细胞毒和溶血等多功能筛选。这些结果表明，这些抗菌肽可能采用复杂的机制，除了抗微生物的主机防御的行动，虽然他们的确切贡献主机防御似乎还不清楚。(C)2011年Elsevier Masson SAS。All rights reserved.
Genus Odorrana, among all amphibians studied, is generally reported to have the most abundant and diversified anti-microbial peptides even from a single individual frog. In our previous work, 46 cDNA sequences encoding precursors of 22 different anti-microbial peptides (AMPs) were characterized from the skin of frog, Odorrana tiannanensis. In this work, we reported the purification of three AMPs from skin secretions of O. tiannanensis. Their amino acid sequences matched well with the sequences deduced from cDNAs and they were designated as Odorranain-C7HSa, Brevinin-1-OT2 and Odorranain-G-OT, respectively. Furthermore, we selected to analyze the four most structurally diversified sequences among the 22 AMPs that are significantly different from all reported AMPs. By structural characterization, three of them were designated as pleurain-E-OT, odorranain-G-OT, odorranain-A-OT, belonging to AMP families already identified. The forth one with a unique 14-mer sequence of AILTTLANWARKFLa and C-terminal amidation represents the prototypes of a new class of amphibian AMP, and thereby named tiannanensin. Such broad diversity in sequences and structures are consistent with other species in Genus Odorrana. Multi-functions of the synthesized four special AMPs were screened, including anti-microbial, antioxidant, cytotoxic and hemolytic activities. The results suggest that these AMPs may employ sophisticated mechanisms of action in host defense in addition to anti-microbial, although their precise contribution to host defense still seems unclear. (C) 2011 Elsevier Masson SAS. All rights reserved.