GENOMIC STRUCTURE OF AN ATTENUATED QUASI-SPECIES OF HIV-1 FROM A BLOOD-TRANSFUSION DONOR AND RECIPIENTS
GENOMIC STRUCTURE OF AN ATTENUATED QUASI-SPECIES OF HIV-1 FROM A BLOOD-TRANSFUSION DONOR AND RECIPIENTS
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DOI：
10.1126/science.270.5238.988
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发表时间：
1995-11-10
期刊：
影响因子：
56.9
通讯作者：
MILLS, J
中科院分区：
文献类型：
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作者：
DEACON, NJ;TSYKIN, A;MILLS, J
一名感染人类免疫缺陷病毒1型（HIV-1）的献血者和6名从该献血者身上感染的血液或血液制品接受者在感染后10至14年仍无HIV-1相关疾病，CD4淋巴细胞计数稳定正常。来自病毒分离株或患者外周血单个核细胞的HIV-1序列在nef基因和长末端重复序列（LTR）的nef和U3区域重叠区域中都有类似的缺失。对一个分离物基因组进行全基因组测序，并对其他队列成员中选定的HIV-1基因组区域进行扩增，未发现其他明显功能意义的异常。这些数据表明，HIV感染后的生存可以由HIV基因组决定，并支持LTR U3区域nef在决定HIV-1致病性中的重要性。
A blood donor infected with human immunodeficiency virus-type 1 (HIV-1) and a cohort of six blood or blood product recipients infected from this donor remain free of HIV-1-related disease with stable and normal CD4 lymphocyte counts 10 to 14 years after infection. HIV-1 sequences from either virus isolates or patient peripheral blood mononuclear cells had similar deletions in the nef gene and in the region of overlap of nef and the U3 region of the long terminal repeat (LTR). Full-length sequencing of one isolate genome and amplification of selected HIV-1 genome regions from other cohort members revealed no other abnormalities of obvious functional significance. These data show that survival after HIV infection can be determined by the HIV genome and support the importance of nef or the U3 region of the LTR in determining the pathogenicity of HIV-1.