Comparison of phospholipase D activity in vasopressin- and phorbol ester-stimulated fibroblasts.
Comparison of phospholipase D activity in vasopressin- and phorbol ester-stimulated fibroblasts.
复制标题
加压素和佛波酯刺激的成纤维细胞中磷脂酶 D 活性的比较。
DOI：
10.1016/0014-5793(93)80054-x
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发表时间：
1993
期刊：
影响因子：
3.5
通讯作者：
Cabot,MC
中科院分区：
文献类型：
--
作者：
Huang,C;Wykle,RL;Cabot,MC
Phospholipase D (PLD) activation by vasopressin (VP) was compared to activation by TPA in REF52 cells prelabeled with [3H]glycerol and [14C]myristic acid. Upon VP-treatment, the formation of [3H] and [14c]phosphatidic acid (PA) and phosphatidylethanol (PEt) was accompanied by the loss of radioactivity from PC and PI. However, upon TPA-treatment, radioactivity was lost from PC only. No significant changes of phosphatidylethanolamine and phosphatidylserine were detected in the same samples. The inclusion of 5μM staurosporine for 10 min diminished the production of [3H]PEt and [14C]PEt by 27% and 53% in VP-treated cells, and by 100% and 75% in TPA-treated cells, respectively. Adding 1 mM EGTA to chelate extracellular Ca2+inhibited [3H]PEt by approximately 31% and [14C]PEt by 17% after VP-stimulation. In contrast, EGTA had no effeet on TPA-stimulation. The data suggest that REF52 cells contain dual PLD activities. The first is stimulated only by VP, requires Ca2+and hydrolyzes PI. The second is stimulated by both TPA and VP, activated by protein kinase C and hydrolyzes PC.
