Effect of Toll-like receptor 4 blockade on pulmonary inflammation caused by Mechanical ventilation and bacterial endotoxin
Effect of Toll-like receptor 4 blockade on pulmonary inflammation caused by Mechanical ventilation and bacterial endotoxin
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DOI：
10.1080/01902140802022492
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发表时间：
2008-06-01
影响因子：
1.7
通讯作者：
Frevert, Charles W.
中科院分区：
文献类型：
--
作者：
Smith, Lincoln S.;Kajikawa, Osamu;Frevert, Charles W.
机械通气（MV）和脂多糖（LPS）协同增加炎症和肺损伤。本研究的目的是确定阻断CD14或toll样受体4 （TLR4）是否会减少LPS引起的炎症。兔经抗TLR4或抗CD14单克隆抗体预处理后，支气管内LPS和MV阻断TLR4可减少支气管肺泡灌洗液中中性粒细胞的数量和CXCL8的数量。相比之下，阻断CD14并没有显著减少中性粒细胞的数量或CXCL8的数量。这些数据表明，阻断TLR4可以减轻LPS和机械通气联合引起的肺部炎症。
Mechanical ventilation (MV) and lipopolysaccharide (LPS) synergistically increase inflammation and lung injury. The goal of this study was to determine whether blockade of CD14 or toll-like receptor 4 (TLR4) would reduce inflammation caused by LPS and MV Rabbits were pretreated with anti-TLR4 or anti-CD14 monoclonal antibodies, followed by endobronchial LPS and MV Blockade of TLR4 reduced the number of neutrophils and the amount of CXCL8 in bronchoalveolar lavage fluid. In contrast, blockade of CD14 did not significantly decrease the number of neutrophils or the amount of CXCL8 These data show that TLR4 blockade reduces pulmonary inflammation caused by the combination of LPS and Mechanical ventilation.