Nanomechanics and Raman spectroscopy of fibrillin 2 knock-out mouse bones
Nanomechanics and Raman spectroscopy of fibrillin 2 knock-out mouse bones
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DOI：
10.1007/s10853-007-1918-x
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发表时间：
2007-11-01
影响因子：
4.5
通讯作者：
Mann, A. B.
中科院分区：
文献类型：
--
作者：
Kavukcuoglu, N. B.;Arteaga-Solis, E.;Mann, A. B.
缺乏骨胶原蛋白2（Fbn 2），一种非胶原性骨蛋白，会导致一种称为先天性挛缩性蛛网膜下腔囊肿（CCA）的结缔组织疾病，并与骨密度降低有关。纳米压痕和拉曼显微光谱已被用来比较Fbn 2-/-缺陷小鼠和野生型对照（Fbn 2 +/+）股骨皮质骨的机械和化学性质。发现与Fbn 2 +/+骨相比，Fbn 2-/-骨具有显著较低的硬度和弹性模量，尤其是在中间皮质部分。拉曼分析显示，除了在Fn 2-/-骨的骨内膜区域的B型碳酸盐取代减少外，基因型之间几乎没有差异。结果表明，Fbn 2在决定骨的力学性能中起着直接的作用。
Absence of fibrillin 2 (Fbn2), a non-collagenous bone protein, causes a connective tissue disorder called congenital contractural arachnodactyly (CCA) and has been associated with decreased bone mineral density. Nanoindentation and Raman microspectroscopy have been used to compare the mechanical and chemical properties of cortical bone from femora of Fbn2-/- deficient mice and their wild-type controls (Fbn2+/+). It was found that Fbn2-/- bones have significantly lower hardness and elastic modulus compared to Fbn2+/+ bones, especially in the mid-cortical section. The Raman analysis showed little difference with genotype except for a decrease in type-B carbonate substitution in the endosteal region of Fbn2-/- bones. The results indicate that Fbn2 plays a direct role in determining the mechanical properties of bone.