Immobilization of Candida antarctica lipase B (CALB) on surface-modified rice husk ashes (RHA) via physical adsorption and cross-linking methods
Immobilization of Candida antarctica lipase B (CALB) on surface-modified rice husk ashes (RHA) via physical adsorption and cross-linking methods
复制标题
DOI：
10.1080/10242422.2016.1247818
复制
发表时间：
2016-07
影响因子：
1.8
通讯作者：
C. Ulker;Nurefsan Gokalp;Y. Guvenilir
中科院分区：
文献类型：
--
作者：
C. Ulker;Nurefsan Gokalp;Y. Guvenilir
摘要本研究采用交联法和吸附法将南极念珠菌脂肪酶B （CALB）固定在表面修饰稻壳灰（RHA）上，其活性回收率均为90%。交联和吸附固定化制剂都非常稳定，在所研究的温度范围内保持了48%以上的活性。两种固定化制剂的最佳温度和最佳pH分别为37℃和7.0℃，交联法和吸附法在4.0℃保存60 d后的相对活性分别为55%和65%。同时，固定化脂肪酶的活性在10个循环后开始下降，两种固定化方法在10个循环后仍保持58%以上的初始活性。这些结果表明，通过交联和吸附固定化脂肪酶不仅可以提高活性恢复，而且可以显著提高稳定性、可重复使用性和应用适应性。结果表明，表面改性的RHA可以作为CALB聚合反应的固定载体。
Abstract In the present study, the recovery of activity of Candida antarctica lipase B (CALB) immobilized onto surface-modified rice husk ash (RHA) was 90% for both cross-linking and adsorption methods. Both cross-linked and adsorbed immobilized preparations were very stable, retaining more than 48% of their activity over the range of temperatures studied. The optimum temperature and optimum pH values were 37 °C and 7.0, respectively for both immobilized preparations, while the relative activities after storage at 4.0 °C for 60 days were 55% and 65% using cross-linking and adsorption methods, respectively. Also, the activity of the immobilized lipase began to decrease after 10 cycles, more than 58% of the initial activities were still retained after 10 cycles for both immobilization methods. These results indicated that lipase immobilized by cross-linking and adsorption not only effected activity recovery, but also remarkably effected stability, reusability and application adaptability. It can be concluded that, surface-modified RHA can be used as alternative supports for immobilization of CALB for polymerization reactions.