A novel immortalized human endometrial stromal cell line with normal progestational response
A novel immortalized human endometrial stromal cell line with normal progestational response
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DOI：
10.1210/en.2003-1606
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发表时间：
2004-05-01
期刊：
影响因子：
4.8
通讯作者：
Lockwood, CJ
中科院分区：
文献类型：
--
作者：
Krikun, G;Mor, G;Lockwood, CJ
获得原代人子宫内膜间质细胞（HESC）的体外研究是有限的，缺乏足够的人的材料和无法通过这些细胞在培养中长时间。这些细胞的永生化将极大地促进研究;然而，永生化的过程往往导致异常核型和异常的功能特征。为了满足这一需要，我们将端粒酶引入培养的HESC中，以防止在有丝分裂期间在成人体细胞中观察到的端粒的正常缩短。我们现在已经开发和分析了一个新的永生化HESC线，不包含克隆染色体结构或数量异常。此外，当与原代未传代的亲本细胞相比时，新细胞系在用卵巢类固醇处理后显示出相似的生化终点。经典的蜕膜化反应，雌二醇加醋酸甲羟孕酮在形态学上，和resistin被认为是诱导或调节IGF结合蛋白-1，纤连蛋白，催乳素，组织因子，纤溶酶原激活物抑制剂-1，Fas/Fas配体的表达。总之，已经开发了一种永生化HESC系，其在核型、形态学和表型上与原代亲本细胞相似，并且其是用于体外工作的强大且一致的资源。
Obtaining primary human endometrial stromal cells (HESCs) for in vitro studies is limited by the scarcity of adequate human material and the inability to passage these cells in culture for long periods. Immortalization of these cells would greatly facilitate studies; however, the process of immortalization often results in abnormal karyotypes and aberrant functional characteristics. To meet this need, we have introduced telomerase into cultured HESCs to prevent the normal shortening of telomeres observed in adult somatic cells during mitosis. We have now developed and analyzed a newly immortalized HESC line that contains no clonal chromosomal structural or numerical abnormalities. In addition, when compared with the primary unpassaged parent cells, the new cell line displayed similar biochemical endpoints after treatment with ovarian steroids. Classical decidualization response to estradiol plus medroxyprogesterone acetate were seen in both morphologically, and progestin was seen to induce or regulate the expression of IGF binding protein-1, fibronectin, prolactin, tissue factor, plasminogen activator inhibitor-1, and Fas/Fas ligand. In summary, an immortalized HESC line has been developed that is karyotypically, morphologically, and phenotypically similar to the primary parent cells, and it is a powerful and consistent resource for in vitro work.