OPN4 belongs to the photosensitive system of the human skin
OPN4 belongs to the photosensitive system of the human skin
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DOI：
10.1111/gtc.12751
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发表时间：
2020-02-06
期刊：
影响因子：
2.1
通讯作者：
Sakakibara, Shunsuke
中科院分区：
文献类型：
--
作者：
Kusumoto, Junya;Takeo, Makoto;Sakakibara, Shunsuke
此前，人类皮肤曾被描述为受光影响；然而，其潜在的机制仍不清楚。Opn4(黑素蛋白)最早是在两栖动物的皮肤中发现的；然而，它是否也在人类皮肤中表达和功能尚未确定。在这里，我们发现OPN4在人类皮肤组织以及分离的角质形成细胞、黑素细胞和成纤维细胞的培养中都有表达。此外，还观察了蓝光照射对人成纤维细胞钙离子内流和细胞外信号调节蛋白1/2的磷酸化的影响。值得注意的是，我们的发现表明，这种钙离子内流和细胞外信号调节激酶1/2的磷酸化以一种强度依赖的方式被促进，这表明光信号在人类皮肤中通过OPN4转换为细胞内信号。总体而言，在这项研究中，我们证明了人类皮肤作为光感受器的功能，证明了在人类皮肤中，光接受蛋白被表达，并且光接收是通过光接受蛋白进行的。
The human skin has previously been described to be affected by light; however, the underlying mechanism remains unknown. OPN4 (melanopsin) expression was first identified in the skin of amphibians; however, whether it is also expressed and functioned in the human skin has not yet been identified. Here, we show that OPN4 was expressed in the human skin tissue and cultures of isolated keratinocytes, melanocytes and fibroblasts. Additionally, Ca2+ influx in vitro and ex vivo and phosphorylation of extracellular signal-regulated kinases 1/2 in human fibroblasts were observed by stimulation of blue light irradiation. Notably, our findings showed that this Ca2+ influx and phosphorylation of extracellular signal-regulated kinases 1/2 are promoted in an intensity-dependent manner, indicating that the light signal is converted to an intracellular signal via OPN4 in the human skin. Overall, in this study we showed that the human skin functions as a photoreceptor by demonstrating that in human skin, the photoreceptive protein was expressed, and photoreception was conducted via photoreceptive protein.