Construction and recovery of viable retroviral genomes carrying a bacterial suppressor transfer RNA gene.
Construction and recovery of viable retroviral genomes carrying a bacterial suppressor transfer RNA gene.
复制标题
携带细菌抑制转移 RNA 基因的可行逆转录病毒基因组的构建和恢复。
DOI：
10.1126/science.2984770
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Goff,SP
中科院分区：
文献类型：
--
作者：
Lobel,LI;Patel,M;King,W;Nguyen-Huu,MC;Goff,SP
The integration of retroviral genomes into cellular DNA can induce mutations by altering the expression of nearby cellular genes and can serve to identify the gene affected. The construction of a retrovirus that stably carries a suppressor transfer RNA gene fromEscherichia colihas allowed facile recovery of the viral genome in vectors marked with amber mutations. This virus can be used for rapid isolation of cellular sequences at the site of proviral insertion.
