Analysis of biochemical compounds and differentially expressed genes of the anthocyanin biosynthetic pathway in variegated peach flowers
Analysis of biochemical compounds and differentially expressed genes of the anthocyanin biosynthetic pathway in variegated peach flowers
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杂色桃花花色苷生物合成途径生化成分及差异表达基因分析
DOI：
10.4238/2015.october.28.4
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发表时间：
2015-01-01
影响因子：
0.4
通讯作者：
Li, S. X.
中科院分区：
文献类型：
--
作者：
Hassani, D.;Liu, H. L.;Li, S. X.
杂色植物在花卉市场上具有很高的价值，但这种吸引人的现象背后的遗传机制尚未完全阐明。本研究采用高效液相色谱和液相色谱/质谱联用技术对桃（Prunus persica）粉红色和白色花瓣中的不同化合物进行了鉴定和测定。在白色花瓣中未检测到基于矢车菊素或基于天竺葵素的化合物，但在粉红色花瓣中发现了高含量的这些化合物。此外，我们测序和分析了花色素苷生物合成途径中的六个关键结构基因（CHI，CHS，DFR，F3 'H，ANS和UFGT）在白色和粉红色花瓣中的表达。定量实时聚合酶链反应显示，所有六个基因的表达水平大大降低，在白色花瓣，相对于粉红色。在转录序列中没有发现等位基因变异。然而，ANS基因的转录序列和基因组序列的比对检测到选择性剪接，导致1.071和942 bp的转录本。在白色花瓣中仅观察到较长的转录本。由于ANS是催化无色的leucopelargonidin和leucocyanidin转化为花青素合成所需底物的关键中间酶，因此ANS基因与花色变异有关，值得进一步研究。本文结合转录组学研究，从花青素合成的关键结构基因方面阐明了桃花色变异的机制。
Variegated plants are highly valuable in the floricultural market, yet the genetic mechanism underlying this attractive phenomenon has not been completely elucidated. In this study, we identified and measured different compounds in pink and white flower petals of peach (Prunus persica) by high-performance liquid chromatography and liquid chromatography/mass spectrometry analyses. No cyanidin-based or pelargonidin-based compounds were detected in white petals, but high levels of these compounds were found in pink petals. Additionally, we sequenced and analyzed the expression of six key structural genes in the anthocyanin biosynthesis pathway (CHI, CHS, DFR, F3'H, ANS, and UFGT) in both white and pink petals. Quantitative real-time polymerase chain reaction revealed all six genes to be expressed at greatly reduced levels in white flower petals, relative to pink. No allelic variations were found in the transcribed sequences. However, alignment of transcribed and genomic sequences of the ANS gene detected alternative splicing, resulting in transcripts of 1.071 and 942 bp. Only the longer transcript was observed in white flower petals. Since ANS is the key intermediate enzyme catalyzing the colorless leucopelargonidin and leucocyanidin to substrates required for completion of anthocyanin biosynthesis, the ANS gene is implicated in flower color variegation and should be explored in future studies. This article, together with a previous transcriptome study, elucidates the mechanism underlying peach flower color variegation in terms of the key structural genes involved in anthocyanin biosynthesis.