The EGFR Protein Expression and the Gene Copy Number Changes in Renal Cell Carcinomas
The EGFR Protein Expression and the Gene Copy Number Changes in Renal Cell Carcinomas
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DOI：
10.4132/koreanjpathol.2009.43.5.413
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发表时间：
2009-10-01
影响因子：
--
通讯作者：
Kim, Insun
中科院分区：
文献类型：
--
作者：
Lee, Sangho;An, Jungsuk;Kim, Insun
背景：表皮生长因子受体（epidermal growth factor receptor，EGFR）参与多种肿瘤的发生发展。EGFR抑制已被尝试作为许多人类恶性肿瘤的治疗方式。研究方法：本文对1995 ~ 1997年间确诊的135例透明细胞癌和16例乳头状肾细胞癌（RCC）中EGFR蛋白表达和基因拷贝数变化进行了研究。结果：EGFR蛋白表达（2+和3+）在透明细胞型RCC中占54.1%，在乳头状RCC中占43.8%。在透明细胞肾癌中，其表达与性别、肿瘤大小（≥ 4cm）、高T分期（T2和T3）有关，具有统计学意义。105例透明细胞肾癌中EGFR基因三体27例（25.7%），多体性40例（38.1%）。三体和多体性与EGFR蛋白表达和高临床T分期相关，具有统计学意义。在15例乳头状肾细胞癌中，13例为三体（86.7%），1例为多体性（6.7%）。透明细胞型和乳头状型肾细胞癌均未见扩增。结论：相当数量的RCC显示EGFR蛋白过表达和EGFR基因拷贝数增加，但在RCC患者中进行FISH研究的临床意义似乎有限。
Background : The epidermal growth factor receptor (EGFR) is known to be involved in many tumor promoting activities. EGFR inhibition has been tried as a therapeutic modality in many human malignancies. Methods : The expression of EGFR protein and the gene copy number changes were studied in 135 clear cell carcinomas and 16 papillary renal cell carcinomas (RCCs), and these tumors were diagnosed between 1995 and 1997. Results : An EGFR protein expression (2+ and 3+) was found in 54.1% of the clear cell RCCs and in 43.8% of the papillary RCCs. In the clear cell RCCs, its expression was associated with male gender, the tumor size (>= 4 cm) and high T stages (T2 and T3), with statistical significance. Trisomy and polysomy of the EGFR gene were found in 27 (25.7%) and 40 (38.1%) of 105 clear cell RCCs, respectively. Trisomy and polysomy were correlated with an EGFR protein expression and a high clinical T stage, with statistical significance. Among 15 papillary RCCs, 13 tumors showed trisomy (86.7%) and one showed polysomy (6.7%). Amplification was not found in both the clear cell and papillary type RCCs. Conclusions : A considerable numbers of RCCs showed an overexpression of EGFR protein and increased EGFR gene copy numbers, yet the clinical significance of conducting a FISH study in RCC patients seems to be limited.