Mapping of magnesium and of different protein fragments in sea urchin teeth via secondary ion mass spectroscopy
Mapping of magnesium and of different protein fragments in sea urchin teeth via secondary ion mass spectroscopy
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DOI：
10.1016/j.jsb.2006.03.002
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发表时间：
2006-07-01
影响因子：
3
通讯作者：
Veis, A.
中科院分区：
文献类型：
--
作者：
Robach, J. S.;Stock, S. R.;Veis, A.
海胆的成熟部分由一系列复杂的增强元素组成，但却是高镁和极高镁方解石的单晶。相对较差的结构材料（方解石）如何能够生产出具有机械性能的结构引起了人们的极大兴趣。在海胆 Lytechinus variegatus 的牙齿中，我们记录了镁、钙以及包括天冬氨酸 (Asp) 在内的矿物质相关蛋白质的特定氨基酸片段的高分辨率二次离子质谱 (SIMS) 图。 SIMS 揭示了 Asp 残基与非常高的 Mg 的强烈共定位。脱矿质标本显示丝氨酸定位在晶体元件之间的膜上，并且镁和天冬氨酸信号减少，进一步强调了非常高的镁与可溶性富含天冬氨酸的蛋白质的共定位。天冬氨酸与非平衡、镁含量极高的方解石的关联为了解参与两种不同成分方解石生长的大分子的构成以及生物矿化的基本过程提供了见解。 (c) 2006 Elsevier Inc. 保留所有权利。
Mature portions of sea urchin are comprised of a complex array of reinforcing elements yet are single crystals of high and very high Mg calcite. How a relatively poor structural material (calcite) can produce mechanically competent structures is of great interest. In teeth of the sea urchin Lytechinus variegatus, we recorded high-resolution secondary ion mass spectrometry (SIMS) maps of Mg, Ca and specific amino acid fragments of mineral-related proteins including aspartic acid (Asp). SIMS revealed strong colocalization of Asp residues with very high Mg. Demineralized specimens showed serine localization on membranes between crystal elements and reduced Mg and aspartic acid signals, further emphasizing colocalization of very high Mg with ready soluble Asp-rich protein(s). The association of Asp with nonequilibrium, very high magnesium calcite provides insight to the makeup of the macromolecules involved in the growth of two different composition calcites and the fundamental process of biomineralization. (c) 2006 Elsevier Inc. All rights reserved.