Lyssaviral infection and lead poisoning in black flying foxes from Queensland
Lyssaviral infection and lead poisoning in black flying foxes from Queensland
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DOI：
10.7589/0090-3558-34.2.355
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发表时间：
1998-04-01
影响因子：
1.3
通讯作者：
Winsor, H
中科院分区：
文献类型：
--
作者：
Skerratt, LF;Speare, R;Winsor, H
在澳大利亚昆士兰州北部的野生黑狐蝠 (Pteropus alecto) 中描述了翼龙狂犬病病毒感染、铅中毒以及通过组织病理学检查诊断翼龙狂犬病病毒感染的困难。 1995年1月，一只成年雌性P. alecto在死亡前表现出攻击性。由于肾近曲小管上皮中存在核内铅包涵体以及肾和肝组织中铅浓度高，诊断为铅中毒。分别为 370.03 +/- 7.35 ppm 和 16.76 +/- 0.53 ppm。肾包涵体由铅、钙、磷和可能的硫组成；有些包裹体的颗粒排列成同心带状。这只蝙蝠的肾组织中也含有中等浓度的镉（8.09 +/- 0.18 ppm）。一只成年雄性 P. alecto 在 1996 年 5 月死亡前出现上行性麻痹。1996 年 9 月，通过脑部狂犬病免疫荧光和免疫过氧化物酶抗体测试以及脑部病毒培养，在两只蝙蝠中诊断出 Pteropid lyssaviral 感染。除了雌性脑干神经元中偶尔出现细微的嗜酸性细胞质内含物外，两只蝙蝠的大脑均未出现表明狂犬病病毒感染的肉眼或微观病变。这些病例说明需要进行特定测试来检测翼龙狂犬病病毒，例如狂犬病病毒的免疫荧光抗体测试，而不是对组织进行组织病理学检查。
Pteropid lyssaviral infection, lead poisoning, and the difficulties in diagnosing pteropid lyssaviral infection using histopathological examination of tissues are described in wild black flying foxes (Pteropus alecto) from northern Queensland (Australia). An adult female P. alecto showed aggression before death in January 1995. Lead poisoning was diagnosed due to the presence of intranuclear lead inclusion bodies in renal proximal convoluted tubular epithelium and high concentrations of lead in renal and hepatic tissues. 370.03 +/- 7.35 ppm and 16.76 +/- 0.53 ppm, respectively. Renal inclusion bodies were composed of lead, calcium, phosphorus, and possibly sulphur; some inclusions had their granules arranged in concentric bands. This bat also had a moderate concentration (8.09 +/- 0.18 ppm) of cadmium in renal tissue. An adult male P. alecto presented with ascending paralysis before it died in May 1996. Pteropid lyssaviral infection was diagnosed subsequently in both bats in September 1996 by immunofluorescent and immunoperoxidase antibody tests for rabies on brains and viral culture from brains. Neither bat had gross or microscopic lesions of the brain that suggested a lyssaviral infection, apart from occasional, subtle, eosinophilic cytoplasmic inclusions in the neurones of the brain stem of the female. These cases illustrate the need for a specific test to detect pteropid lyssavirus such as an immunofluorescent antibody test for lyssavirus rather than histopathological examination of tissues.