Pathological characterization of Kank in renal cell carcinoma
Pathological characterization of Kank in renal cell carcinoma
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DOI：
10.1016/j.yexmp.2004.08.012
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发表时间：
2005-02-01
影响因子：
3.6
通讯作者：
Kiyama, R
中科院分区：
文献类型：
--
作者：
Roy, BC;Aoyagi, T;Kiyama, R
Kank基因是通过杂合性缺失搜索在肾细胞癌(RCC)中发现的9p24位候选抑癌基因，并且可能通过肌动蛋白的聚合来控制细胞骨架的形成。在这里，我们用抗Kank的单抗鉴定了肾小管细胞以及结肠、胃、前列腺、睾丸、胰腺、甲状腺、子宫、颌下腺、肾上腺、十二指肠和食道中的其他腺细胞以及肝、肺泡心肌和神经胶质细胞等特定细胞中的Kank蛋白。免疫组织化学和Western印迹分析发现1例肾细胞癌组织中Kank基因表达缺失，CDKN2a(p16/Ink4a)基因表达保留。用荧光免疫组织化学方法检测Kank在HEK293和VMRC-RCW细胞以及原代培养的肾小管上皮细胞胞浆和膜褶皱部位的表达。(C)2004 Elsevier Inc.保留所有权利。
The Kank gene was found as a candidate tumor suppressor gene at 9p24 by loss-of-heterozygosity search in renal cell carcinoma (RCC) and seems to have a role in controlling the formation of the cytoskeleton through the polymerization of actin. Here, we characterized the Kank protein in renal tubular cells as well as other glandular cells in the colon, stomach, prostate, testis, pancreas, thyroid, uterus, submandibular gland, adrenal, duodenum, and esophagus, and specific cells such as hepatic, alveolar myocardial, and glial cells by using a monoclonal antibody against Kank. Loss of expression of Kank in one RCC sample was detected by immunohistochemical and Western blot analyses while expression of CDKN2A (p16/Ink4A) was retained in the sample. The expression of Kank in the cytoplasm and at the sites of membrane ruffling in HEK293 and VMRC-RCW cells and in a primary culture of renal tubular cells was also detected by fluorescence-based immunostaining. (C) 2004 Elsevier Inc. All rights reserved.