Optical aberrations in the mouse eye
Optical aberrations in the mouse eye
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DOI：
10.1016/j.visres.2006.01.011
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发表时间：
2006-08-01
期刊：
影响因子：
1.8
通讯作者：
Marcos, Susana
中科院分区：
文献类型：
--
作者：
de la Cera, Elena Garcia;Rodriguez, Guadalupe;Marcos, Susana
目的：小鼠眼是一种广泛应用的视网膜疾病模型，有可能成为近视模型。视网膜疾病的研究将受益于眼底的活体成像。近视的实验模型往往依赖于对视觉经验的操纵。在这两种情况下，眼睛的光学质量的知识，特别是，视网膜图像质量的退化强加的眼像差是必不可少的。在这项研究中，我们测量了野生型小鼠的眼像差。方法：对6只4周龄黑色C57BL/6野生型小鼠的12只眼进行研究。测量是在清醒的动物上进行的，为了比较起见，其中一只动物在麻醉下也进行了测量。使用特制的Hartmann-Shack系统（使用680 nm照明）测量眼像差。波像差被报道到四阶泽尼克多项式。由二阶泽尼克项得到球面等效和像散。估计调制传递函数（MTF）为最佳焦点，并通过焦点，以估计焦深。所有报告的数据均为1.5毫米瞳孔。结果：Hartmann-Shack屈光持续远视（10.12 +/- 1.41 D，平均值和标准差），多只眼存在散光（平均3.64 +/- 3.70 D）。所有眼睛的球差均为正（0.15 +/- 0.07 Pin），彗差项的均方根显著高于其他泽尼克项（0.10 +/- 0.03 μ m）。从波像差估计的MTFs显示，在2℃/°时，调制为0.4，以获得最佳聚焦（在不抵消测量的离焦的情况下调制为0.15）。对于该空间频率，使用瑞利标准从透焦调制数据估计的焦深为6 d，一只麻醉小鼠的眼睛的像差高于清醒动物的同只眼睛。结论：小鼠眼睛的远视屈光与先前的视网膜检查数据一致。老鼠眼睛的光学远没有在1.5毫米瞳孔处受到衍射限制，有大量的球差和彗差。然而，波像差对mtf的估计比以前使用双通道技术报道的要高，导致较小的景深预测。尽管像差造成了很大的退化，但这些像差通常比可用的校正技术（即自适应光学）校正的像差量要低。另一方面，像差似乎不是鼠标空间分辨率的限制因素。虽然小鼠的光学比其他实验近视模型退化得更严重，但其对大量离焦的耐受性似乎并不完全由眼像差决定。(c) 2006 Elsevier Ltd.版权所有。
Purpose: The mouse eye is a widely used model for retinal disease and has potential to become a model for myopia. Studies of retinal disease will benefit from imaging the fundus in vivo. Experimental models of myopia often rely on manipulation of the visual experience. In both cases, knowledge of the optical quality of the eye, and in particular, the retinal image quality degradation imposed by the ocular aberrations is essential. In this study, we measured the ocular aberrations in the wild type mouse.Methods: Twelve eyes from six four-week old black C57BL/6 wild type mice were studied. Measurements were done on awake animals, one being also measured under anesthesia for comparative purposes. Ocular aberrations were measured using a custom-built Hartmann-Shack system (using 680-nm illumination). Wave aberrations are reported up to fourth order Zernike polynomials. Spherical equivalent and astigmatism were obtained from the 2nd order Zernike terms. Modulation Transfer Functions (MTF) were estimated for the best focus, and through-focus, to estimate depth-of-focus. All reported data were for 1.5-mm pupils.Results: Hartmann-Shack refractions were consistently hyperopic (10.12 +/- 1.41 D, mean and standard deviation) and astigmatism was present in many of the eyes (3.64 +/- 3.70 D, on average). Spherical aberration was positive in all eyes (0.15 +/- 0.07 Pin) and coma terms RMS were significantly high compared to other Zernike terms (0.10 +/- 0.03 mu m). MTFs estimated from wave aberrations show a modulation of 0.4 at 2 c/deg, for best focus (and 0.15 without cancelling the measured defocus). For that spatial frequency, depth-of-focus estimated from through-focus modulation data using the Rayleigh criterion was 6 D. Aberrations in the eye of one anesthetized mouse were higher than in the same eye of the awake animal.Conclusions: Hyperopic refractions in the mouse eye are consistent with previous retinoscopic data. The optics of the mouse eye is far from being diffraction-limited at 1.5-mm pupil, with significant amounts of spherical aberration and coma. However, estimates of MTFs from wave aberrations are higher than previously reported using a double-pass technique, resulting in smaller depth-of-field predictions. Despite the large degradation imposed by the aberrations these are lower than the amount of aberrations typically corrected by available correction techniques (i.e., adaptive optics). On the other hand, aberrations do not seem to be the limiting factor in the mouse spatial resolution. While the mouse optics are much more degraded than in other experimental models of myopia, its tolerance to large amounts of defocus does not seem to be determined entirely by the ocular aberrations. (c) 2006 Elsevier Ltd. All rights reserved.