Development of a liquid phase immunoassay by time-dependent measurements of the transient magneto-optical birefringence using functionalized magnetic nanoparticles
Development of a liquid phase immunoassay by time-dependent measurements of the transient magneto-optical birefringence using functionalized magnetic nanoparticles
复制标题
使用功能化磁性纳米颗粒对瞬态磁光双折射进行时间依赖性测量，开发液相免疫测定法
DOI：
10.1016/j.jmmm.2004.11.135
复制
发表时间：
2005
影响因子：
2.7
通讯作者：
W. Weitschies
中科院分区：
文献类型：
--
作者：
G. Glöckl;V. Brinkmeier;K. Aurich;E. Romanus;P. Weber;W. Weitschies
瞬态磁光双折射是一种新的液相免疫分析技术。研究了使用单克隆抗体和多克隆抗体的特异性结合磁性探针。确定了优化的磁性纳米粒子的检测限。多克隆探针与其特异性抗原的结合实验产生了增加的颗粒尺寸，如从磁光弛豫曲线所确定的。由于交联引起的磁性探针聚集导致信号强度逐渐降低。
The transient magneto-optical birefringence was investigated as a new liquid phase immunoassay technique. Specifically binding magnetic probes using monoclonal as well as polyclonal antibodies were investigated. The detection limit for optimized magnetic nanoparticles was determined. Binding experiments of polyclonal probes with their specific antigen yielded increased particle sizes as determined from magneto-optical relaxation curves. Aggregation of magnetic probes due to cross-linking caused gradually decreased signal intensity.