Molecular cloning and functional expression of a potassium channel cDNA isolated from a rat cardiac library
Molecular cloning and functional expression of a potassium channel cDNA isolated from a rat cardiac library
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DOI：
10.1016/0014-5793(90)80973-m
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发表时间：
1990-07
期刊：
影响因子：
3.5
通讯作者：
J. Tseng-Crank;G. Tseng;A. Schwartz;M. Tanouye
中科院分区：
文献类型：
--
作者：
J. Tseng-Crank;G. Tseng;A. Schwartz;M. Tanouye
以果蝇ShakerH 4和小鼠脑MBK 1的K+通道保守序列为模板，采用聚合酶链反应（PCR）方法，从大鼠心肌细胞库中扩增出K+通道全长cDNA（RHK 1）。虽然RHK 1是从心脏中分离出来的，但它在心脏和脑中都有表达。RHK 1编码的蛋白质在异种卵母细胞中表达时，门控4-氨基吡啶（4-AP）敏感的瞬时外向电流。该电流与大鼠心室肌细胞中测得的瞬时外向电流在激活和失活的电压依赖性、激活和失活的时程以及药理学方面相似。
A full-length K+channel cDNA (RHK1) was isolated from a rat cardiac library using the polymerase chain reaction (PCR) method and degenerate oligonucleotide primers derived from K+channel sequences conserved betweenDrosophila ShakerH4 and mouse brain MBK1. Although RHK1 was isolated from heart, its expression was found in both heart and brain. The RHK1-encoded protein, when expressed inXenopusoocytes, gated a 4-aminopyridine (4-AP)-sensitive transient outward current. This current is similar to the transient outward current measured in rat ventricular myocytes with respect to voltage-dependence of activation and inactivation, time course of activation and inactivation, and pharmacology.