Microsatellite analysis reveals genetic structure of Leishmania tropica
Microsatellite analysis reveals genetic structure of Leishmania tropica
复制标题
DOI：
10.1016/j.ijpara.2005.09.010
复制
发表时间：
2006-02-01
影响因子：
4
通讯作者：
Schönian, G
中科院分区：
文献类型：
--
作者：
Schwenkenbecher, JM;Wirth, T;Schönian, G
目前热带利什曼原虫引起的利什曼病的迅速传播及其临床谱的复杂性要求对这种寄生虫进行流行病学和进化调查。用同工酶电泳和先前的分子生物学分析来评价其种群结构是困难的。在这项研究中，我们使用21个微卫星位点对来自非洲和亚洲不同地区的117株菌株进行了分型。发现了81种不同的基因型。等位基因数量梯度支持的遗传瓶颈与中东的地理结构一致，表明该物种起源于非洲。贝叶斯方法确定了10个主要与地理起源相关的遗传集群。“亚洲”集群中的菌株形成了一个非常异质的亚种群，具有不同但相互关联的基因型，在地理上分布非常广泛，与寄生虫的人传传播相一致。其他9个集群更为同质化。热带L.的繁殖似乎以无性系为主。在非洲和中东，人传和人畜共患的分布系统可能有助于热带乳杆菌重叠的、遗传上不同的种群的发展。(C) 2005澳大利亚寄生虫学学会Elsevier Ltd.出版。版权所有。
The current rapid spread of leishmaniases caused by Leishmania tropica and the complexity of its clinical spectrum call for this parasite's epidemiological and evolutionary investigation. Evaluation of its population structure by isoenzyme electrophoresis and previous molecular biological analysis has proved difficult. In this study, we used 21 microsatellite loci to type 117 strains from different African and Asian locations. Eighty-one different genotypes were found. A genetic bottleneck supported by a gradient in the number of alleles and consistent with the geographical structure of the Middle East suggests an African origin of this species. A Bayesian approach identified 10 genetic clusters that correlated predominantly with geographical origin. The strains in the 'Asia' cluster form a very heterogeneous sub-population, with a varied but inter-related genotype that is geographically very widely dispersed and consistent with anthroponotic transmission of the parasite. The other nine clusters were more homogenous. The propagation of L. tropica appears to be predominantly clonal. In Africa and the Middle East, anthroponotic and zoonotic systems of distribution may contribute to the development of overlapping, genetically distinct populations of L. tropica. (C) 2005 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.