High resolution electron paramagnetic resonance imaging of biological samples with a single line paramagnetic label
High resolution electron paramagnetic resonance imaging of biological samples with a single line paramagnetic label
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DOI：
10.1002/mrm.1910370402
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发表时间：
1997-04-01
影响因子：
3.3
通讯作者：
Zweier, JL
中科院分区：
文献类型：
--
作者：
Kuppusamy, P;Wang, PH;Zweier, JL
电子顺磁共振成像（EPRI）从生物样品中获得信息的应用受到缺乏理想的单线自由基标记的限制。常用的氮氧化物表现出多条线，导致在最大可获得的图像分辨率中的基于超精细的限制或在重建图像中的基于超精细的伪影。使用一种新的单线三芳基甲基顺磁标记，使显着提高图像质量和分辨率的报告。该标记物显示出单线EPR光谱，该光谱在组织中是尖锐的（在不存在氧的情况下，线宽类似于60 mG）并且相对稳定。在一系列的幻影和分离的生物器官，如大鼠肾脏，该标签在使生物样品的高分辨率EPR成像的潜力得到证明，该图像表明，分辨率优于100 μ m，可以在L波段上获得的样品的大小为20毫米。
The application of electron paramagnetic resonance imaging (EPRI) to obtain information from biological samples has been limited by the lack of ideal single line radical labels, The commonly used nitroxides exhibit multiple lines causing either hyperfine-based limitations in the maximum obtainable image resolution or hyperfine-based artifacts in the reconstructed image. The use of a novel single-line triarylmethyl paramagnetic label that enables marked enhancement in image quality and resolution is reported. This label exhibits a single line EPR spectrum that is sharp (linewidth similar to 60 mG in the absence of oxygen) and relatively stable in tissues. The potential of this label in enabling high resolution EPR imaging of biological samples was demonstrated in a series of phantoms and isolated biological organs such as the rat kidney, The images demonstrate that resolutions better than 100 mu m could be obtained at L-band on samples of up to 20 mm in size.