G-quadruplex dynamics
G-quadruplex dynamics
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DOI：
10.1016/j.bbapap.2017.06.012
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发表时间：
2017-11-01
影响因子：
3.2
通讯作者：
Mittermaier, Anthony K.
中科院分区：
文献类型：
--
作者：
Harkness, Robert W., V;Mittermaier, Anthony K.
G-四链体（GQs）是由富含鸟苷（G）的DNA和RNA序列形成的四链核酸二级结构。越来越清楚的是，包括基因表达和mRNA翻译在内的细胞过程受到GQ的调控。GQ结构已被广泛表征，然而迄今为止很少关注它们的构象动力学，尽管许多生物GQ序列占据具有相似自由能的多个结构，导致交换构象的集合。这些动力学对生物功能的影响目前还不清楚。最近，结构动力学已被证明熵稳定GQ合奏，潜在地调节基因表达。GQ系综中的瞬时、低密度状态可以另外调节核酸相互作用和功能。这篇综述将强调GQ动力学和生物功能的相互作用，重点是生物GQ的几个动态过程和GQ动力学的表征与其他生物物理技术结合的核磁共振（NMR）光谱。这篇文章是由刘易斯凯、约翰班齐格、阿尔伯特伯吉斯和彼得蒂尔曼编辑的《加拿大生物物理学》特刊的一部分。
G-quadruplexes (GQs) are four-stranded nucleic acid secondary structures formed by guanosine (G)-rich DNA and RNA sequences. It is becoming increasingly clear that cellular processes including gene expression and mRNA translation are regulated by GQs. GQ structures have been extensively characterized, however little attention to date has been paid to their conformational dynamics, despite the fact that many biological GQ sequences populate multiple structures of similar free energies, leading to an ensemble of exchanging conformations. The impact of these dynamics on biological function is currently not well understood. Recently, structural dynamics have been demonstrated to entropically stabilize GQ ensembles, potentially modulating gene expression. Transient, low-populated states in GQ ensembles may additionally regulate nucleic acid interactions and function. This review will underscore the interplay of GQ dynamics and biological function, focusing on several dynamic processes for biological GQs and the characterization of GQ dynamics by nuclear magnetic resonance (NMR) spectroscopy in conjunction with other biophysical techniques. This article is part of a Special Issue entitled: Biophysics in Canada, edited by Lewis Kay, John Baenziger, Albert Berghuis and Peter Tieleman.