Multiple pathways mediate the sex-peptide-regulated switch in female Drosophila reproductive behaviours
Multiple pathways mediate the sex-peptide-regulated switch in female Drosophila reproductive behaviours
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DOI：
10.1098/rspb.2013.1938
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发表时间：
2013-11-22
影响因子：
4.7
通讯作者：
Soller, Matthias
中科院分区：
文献类型：
--
作者：
Haussmann, Irmgard U.;Hemani, Yash;Soller, Matthias
雄性性肽（SP）诱导果蝇雌性行为的深刻变化，导致进一步交配的接受性降低和产卵增加。SP可以通过G蛋白偶联受体（SPR）介导雌性生殖行为的转换，SPR位于表达无结果、doublemex和pickpocket的神经元中。据我们所知，SPR是否是唯一的受体以及SP是否在单一途径中诱导交配后转换还没有被测试。在这里，我们报告，SP反应可以诱导在SPR的情况下，当SP异位表达在神经元或SP，通过交配转移，可以通过渗漏的血脑屏障进入神经元。膜栓系SP可以诱导产卵通过douspleex，但不无果和扒窃神经元在SPR突变体的女性。虽然pickpocket神经元和doublesex神经元依赖于G（o）信号传导来降低感受性和诱导产卵，但无果实神经元中的G（o）信号传导仅需要诱导产卵，而不需要降低感受性。结果表明，SP降低感受性和诱导产卵的作用在无果神经元和无果神经元中是分开的。因此，SP诱导的交配后开关包括共享的，但也是不同的电路的无果，doubledex和扒手神经元和额外的受体。
Male-derived sex-peptide (SP) induces profound changes in the behaviour of Drosophila females, resulting in decreased receptivity to further mating and increased egg laying. SP can mediate the switch in female reproductive behaviours via a G protein-coupled receptor, SPR, in neurons expressing fruitless, doublesex and pickpocket. Whether SPR is the sole receptor and whether SP induces the postmating switch in a single pathway has not, to our knowledge been tested. Here we report that the SP response can be induced in the absence of SPR when SP is ectopically expressed in neurons or when SP, transferred by mating, can access neurons through a leaky blood brain barrier. Membrane-tethered SP can induce oviposition via doublesex, but not fruitless and pickpocket neurons in SPR mutant females. Although pickpocket and doublesex neurons rely on G(o) signalling to reduce receptivity and induce oviposition, G(o) signalling in fruitless neurons is required only to induce oviposition, but not to reduce receptivity. Our results show that SP's action in reducing receptivity and inducing oviposition can be separated in fruitless and doublesex neurons. Hence, the SP-induced postmating switch incorporates shared, but also distinct circuitry of fruitless, doublesex and pickpocket neurons and additional receptors.