Coordination of Cl- secretion and contraction by a histamine H2-receptor agonist in guinea pig distal colon.
Coordination of Cl- secretion and contraction by a histamine H2-receptor agonist in guinea pig distal colon.
复制标题
组胺 H2 受体激动剂在豚鼠远端结肠中协调 Cl- 分泌和收缩。
DOI：
10.1152/ajpgi.1993.265.5.g973
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Rogers,R
中科院分区：
文献类型：
--
作者：
Cooke,HJ;Wang,YZ;Rogers,R
在豚鼠远端结肠全厚段浆膜表面缝合应变片，同时测量短路电流(ISC)，同时记录纵轴或周轴的肌张力。ISC和小幅时相性收缩的幅度和频率在数小时内稳定。组胺H2受体激动剂迪马普利特(5-10微米)以0.32-0.37次/分钟的频率诱发ISC的复发性增加。与每一个周期性的ISC反应相关的是肌肉张力的增加，这表明大幅度的相性收缩。10微米的西咪替丁可消除ISC的复发周期和相关的大幅度时相收缩。在迪马普利特存在的情况下，切断肌间神经丛和粘膜下神经丛之间的神经联系可以消除大幅度的相性收缩，但不能消除周期性的ISC。结果表明，组胺H2受体慢性激活过程中周期性分泌和大幅度收缩的协调需要黏膜下神经丛和肌间神经丛之间完整的神经联系。这些发现提供了潜在的机制来解释炎症性疾病或对食物抗原的过敏反应过程中运动性和离子运输的变化。
Short-circuit current (Isc) was measured simultaneously with muscle tension recorded in the longitudinal or circumferential axis from strain-gauge transducers sutured to the serosal surface of whole thickness segments of distal colon from guinea pigs. Isc and the amplitude and frequency of small-amplitude phasic contractions were stable for several hours. The histamine H2-receptor agonist dimaprit (5-10 microM) evoked recurrent increases in Isc at a frequency of 0.32-0.37 cycles/min. Associated with each cyclical Isc response was an increase in muscle tension indicative of large-amplitude phasic contractions. Recurrent cycles of Isc and associated large-amplitude phasic contractions were abolished by 10 microM cimetidine. In the presence of dimaprit, severing the neural connections between the myenteric and submucosal plexuses abolished the large-amplitude phasic contractions but not cyclical Isc. The results suggest that coordination of cyclical secretion and large-amplitude contraction during chronic activation of histamine H2 receptors requires intact neural connections between the submucosal and myenteric plexuses. The findings provide potential mechanisms to explain altered motility and ion transport during inflammatory diseases or allergic responses to food antigens.