Recognition of nucleic acid double helices by homopyrimidine 2′,5′-linked RNA
Recognition of nucleic acid double helices by homopyrimidine 2′,5′-linked RNA
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DOI：
10.1093/nar/26.22.5152
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发表时间：
1998-11-15
影响因子：
14.9
通讯作者：
Noronha, A
中科院分区：
文献类型：
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作者：
Damha, MJ;Noronha, A
我们研究了双链DNA、双链RNA和DNA/RNA杂交体中2 ′，5 ′-RNA第三链骨架对嘧啶基序靶向多嘌呤链的三螺旋稳定性的影响。采用Roberts和Crothers的实验设计，以DNA和区域异构体RNA作为第三链进行了平行的比较实验。结果表明，2 ′，5 ′-RNA第三链骨架对嘧啶基序靶向多嘌呤链的三螺旋稳定性的影响，5 '-RNA确实能够识别双螺旋DNA（DD）和DNA（嘌呤）：RNA然而，当双链嘌呤链是RNA并且双链嘧啶链是DNA或RNA时，基于T-m数据，2 '，5'-RNA和DNA第三链对DD和DR双链体的亲和力是相似的。RNA第三链与所有四个发夹形成三链体，如前所述，类似于阿拉伯糖和2 ′-脱氧核糖第三链，2 ′，5 ′-连接的核糖的可能的C2 ′-内折叠以及α-2 ′-OH基团的缺乏被认为是2 ′，5 ′-RNA相对于RR和RD双链体选择性结合DD和DR双链体的原因，这些研究表明，使用其他寡核苷酸类似物将证明是非常有用的解剖的贡献的骨干和/或糖puckering的核酸双链体的识别。
We have studied the effect of a 2',5'-RNA third strand backbone on the stability of triple helices with a 'pyrimidine motif' targeting the polypurine strand of duplex DNA, duplex RNA and DNA/RNA hybrids, Comparative experiments were run in parallel with DNA and the regioisomeric RNA as third strands adopting the experimental design of Roberts and Crothers,The results reveal that 2',5'-RNA is indeed able to recognize double helical DNA (DD) and DNA (purine):RNA (pyrimidine) hybrids (DR), However, when the duplex purine strand is RNA and the duplex pyrimidine strand is DNA or RNA (i.e. RD or RR), tripler formation is not observed, These results exactly parallel what is observed for DNA third strands, Based on T-m data, the affinities of 2',5'-RNA and DNA third strands towards DD and DR duplexes were similar, The RNA third strand formed triplexes with all four hairpins, as previously demonstrated, In analogy to the arabinose and 2'-deoxyribose third strands, the possible C2'-endo pucker of 2',5'-linked riboses together with the lack of an alpha-2'-OH group are believed to be responsible for the selective binding of 2',5'-RNA to DD and DR duplexes, over RR and RD duplexes, These studies indicate that the use of other oligonucleotide analogues will prove extremely useful in dissecting the contributions of backbone and/or sugar puckering to the recognition of nucleic acid duplexes.