Structural transitions of a twisted and stretched DNA molecule
Structural transitions of a twisted and stretched DNA molecule
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DOI：
10.1103/physrevlett.83.1066
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发表时间：
1999-08-02
影响因子：
8.6
通讯作者：
Marko, JF
中科院分区：
文献类型：
--
作者：
Léger, JF;Romano, G;Marko, JF
我们报告了在力高达150 pN的单双螺旋DNA分子的微操作研究结果。根据DNA缠绕是否允许放松或保持固定，可以观察到不同性质的结构转变。通过研究作为缠绕功能的过渡，表征了它们背后的不同DNA结构；这允许我们报告S-DNA螺旋度的第一个估计。引入了一个模型来描述这些转换；除了B-DNA外，我们还发现需要四种DNA状态来描述实验。
We report results of a micromanipulation study of single double-helical DNA molecules at forces up to 150 pN. Depending on whether the DNA winding is allowed to relax, or held fixed, qualitatively different structural transitions are observed. By studying the transitions as a function of winding the different DNA structures underlying them are characterized; this allows us to report the first estimate of S-DNA helicity. A model is introduced to describe these transitions; in addition to B-DNA, we find that four DNA states are needed to describe the experiments.