Hantaan virus induced elevation of plasma osteoprotegerin and its clinical implications in hemorrhagic fever with renal syndrome
Hantaan virus induced elevation of plasma osteoprotegerin and its clinical implications in hemorrhagic fever with renal syndrome
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汉滩病毒引起肾综合征出血热血浆护骨素升高及其临床意义
DOI：
10.1016/j.ijid.2022.11.005
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Ying Ma
中科院分区：
文献类型：
--
作者：
Kang Tang;Yusi Zhang;Chunmei Zhang;Haifeng Hu;Ran Zhuang;Boquan Jin;Yun Zhang;Ying Ma
目的：出血倾向是汉滩病毒(HTNV)感染后肾综合征出血热(HFRS)的一个标志。越来越多的研究表明护骨素(OPG)在血管内稳态中的重要性，提示OPG可能参与了肾综合征出血热(HFRS)患者凝血功能紊乱的发病机制。采用酶联免疫吸附试验(EL ISA)检测血浆OPG水平等指标。用HTNV和/或肿瘤坏死因子-α刺激人脐静脉内皮细胞，以探讨OPG的来源。结果：肾综合征出血热患者血浆OPG水平升高，并与病情严重程度呈正相关，与血小板计数呈负相关。随着HTNV感染，血管内皮细胞释放大量的OPG，这与血浆OPG与肿瘤坏死因子-α或C反应蛋白呈正相关的结果一致。重要的是，血浆OPG水平与活化部分凝血活酶时间和D-二聚体含量呈正相关。结论：HTNV诱导的血浆OPG水平升高可能是影响HFRS病情严重程度的重要因素，可能参与了HFRS的内皮功能障碍和出血性疾病，可能参与了HFRS出血的发病机制。
Objectives: The bleeding tendency is a hallmark of hemorrhagic fever with renal syndrome (HFRS) after Hantaan virus (HTNV) infection. Growing reports indicate the importance of osteoprotegerin (OPG) in vascular homeostasis, implying OPG might be involved in the pathogenesis of coagulopathy in patients with HFRS...Methods: Acute and convalescence plasmas of 32 patients with HFRS were collected. Enzyme-linked immunosorbent assays (ELISA) were used to detect plasma OPG levels and other parameters. The human umbilical vein endothelial cells were stimulated with HTNV and/or tumor necrosis factor-α (TNF-α) to explore the source of OPG...Results: Plasma OPG levels of patients with HFRS were elevated and correlated positively with the severity of HFRS and negatively with platelet counts. Abundant OPG was released from endothelial cells in response to TNF-α stimuli, along with HTNV infection, which was in accordance with the findings of positive correlations between plasma OPG and TNF-α or c-reactive protein. Importantly, plasma OPG levels correlated positively with activated partial thromboplastin time and the content of d-dimer...Conclusion: These findings suggested that increased plasma OPG levels induced by HTNV might be an important factor for the severity of HFRS, and was likely involved in endothelium dysfunction and hemorrhagic disorder of HFRS, which might contribute to the pathogenesis of hemorrhage in HFRS.