Characterization of a grapevine R2R3-MYB transcription factor that regulates the phenylpropanoid pathway
Characterization of a grapevine R2R3-MYB transcription factor that regulates the phenylpropanoid pathway
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DOI：
10.1104/pp.105.067231
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发表时间：
2006-02-01
期刊：
影响因子：
7.4
通讯作者：
Hamdi, S
中科院分区：
文献类型：
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作者：
Deluc, L;Barrieu, F;Hamdi, S
葡萄（葡萄属vinifera）果实成熟的特征在于基因表达、酶活性和代谢的显著变化，这些变化导致果实品质所必需的化合物的产生。苯丙烷代谢途径是参与这些变化的组分之一。在这项研究中，我们描述了VvMYB 5a的克隆和功能特性，从葡萄L。cv赤霞珠浆果文库。VvMYB 5a编码属于R2 R3-MYB转录因子小亚家族的蛋白质。葡萄藤中的表达研究表明，VvMYB 5a基因主要在浆果发育的早期阶段在皮肤、果肉和种子中表达。VvMYB 5a在烟草中的过表达影响了控制苯丙素合成的结构基因的表达，并影响了花青素、黄酮醇、单宁和木质素的代谢。过量表达VvMYB 5a诱导了几种酚类化合物的强烈积累，包括角花青苷（矢车菊素-3-鼠李糖葡萄糖苷）和槲皮素-3-鼠李糖葡萄糖苷，它们是烟草中主要的花青素和黄酮醇化合物。此外，VvMYB 5a过表达增加了缩合单宁的生物合成并改变了木质素代谢。这些发现表明，VvMYB 5a可能参与控制葡萄藤中苯丙烷途径的不同分支。
The ripening of grape (Vitis vinifera) berry is characterized by dramatic changes in gene expression, enzymatic activities, and metabolism that lead to the production of compounds essential for berry quality. The phenylpropanoid metabolic pathway is one of the components involved in these changes. In this study, we describe the cloning and functional characterization of VvMYB5a, a cDNA isolated from a grape L. cv Cabernet Sauvignon berry library. VvMYB5a encodes a protein belonging to a small subfamily of R2R3-MYB transcription factors. Expression studies in grapevine indicate that the VvMYB5a gene is mainly expressed during the early steps of berry development in skin, flesh, and seeds. Overexpression of VvMYB5a in tobacco ( Nicotiana tabacum) affects the expression of structural genes controlling the synthesis of phenylpropanoid and impacts on the metabolism of anthocyanins, flavonols, tannins, and lignins. Overexpressing VvMYB5a induces a strong accumulation of several phenolic compounds, including keracyanin (cyanidin-3-rhamnoglucoside) and quercetin-3-rhamnoglucoside, which are the main anthocyanin and flavonol compounds in tobacco. In addition, VvMYB5a overexpression increases the biosynthesis of condensed tannins and alters lignin metabolism. These findings suggest that VvMYB5a may be involved in the control of different branches of the phenylpropanoid pathway in grapevine.