The fission yeast synaptobrevin ortholog Syb1 plays an important role in forespore membrane formation and spore maturation.
The fission yeast synaptobrevin ortholog Syb1 plays an important role in forespore membrane formation and spore maturation.
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裂殖酵母突触短蛋白直系同源物 Syb1 在前孢子膜形成和孢子成熟中发挥重要作用。
DOI：
10.1128/ec.00061-13
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
Taro Nakamura (T. Y. and K. I. contributed equally to this article)
中科院分区：
文献类型：
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作者：
Tomomi Yamaoka;Kazuki Imada;Kana Fukunishi;Yuriko Yamasaki;Chikashi Shimoda;Taro Nakamura (T. Y. and K. I. contributed equally to this article)
小泡突触蛋白（Synaptobrevin），又称囊泡相关膜蛋白（VAMP），是质膜N-甲基马来酰亚胺敏感因子附着蛋白受体（SNARE）复合物的一个组成部分，在细胞内膜融合中起关键作用。以前的研究表明，类似于其他生物体中的小突触泡蛋白，裂殖酵母小突触泡蛋白直系同源物Syb 1与高尔基体后分泌囊泡相关联，并且对于胞质分裂和细胞伸长至关重要。在这里，我们报告Syb 1在孢子形成中发挥作用。氮饥饿后，在细胞内斑点中发现绿色荧光蛋白（GFP）-Syb 1。随着减数分裂的进行，GFP-Syb 1在细胞核周围积累，然后定位于前孢子膜（FSM）。我们分离到了asyb-S1突变体，该突变体在孢子形成方面表现出缺陷。在syb 1-S1突变体中，FSM开始形成，但未能发育成正常形态。电子显微镜观察发现syb 1-S1突变体的孢子常形成异常孢子壁。虽然大多数syb 1-S1突变体孢子萌发，但它们对乙醇的耐受性低于野生型孢子。Thesyb 1-S1等位基因携带错义突变，导致跨膜结构域附近保守的半胱氨酸残基被替换，这降低了Syb 1蛋白的稳定性和丰度。综上所述，这些结果表明Syb 1在FSM组装和孢子壁形成中起着重要作用。
Synaptobrevin, also called vesicle-associated membrane protein (VAMP), is a component of the plasma membrane N-methylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, which plays a key role in intracellular membrane fusion. Previous studies have revealed that, similar to synaptobrevin in other organisms, the fission yeast synaptobrevin ortholog Syb1 associates with post-Golgi secretory vesicles and is essential for cytokinesis and cell elongation. Here, we report that Syb1 has a role in sporulation. After nitrogen starvation, green fluorescent protein (GFP)-Syb1 is found in intracellular dots. As meiosis proceeds, GFP-Syb1 accumulates around the nucleus and then localizes at the forespore membrane (FSM). We isolated asyb-S1mutant, which exhibits a defect in sporulation. Insyb1-S1mutants, the FSM begins to form but fails to develop a normal morphology. Electron microscopy shows that an abnormal spore wall is often formed insyb1-S1mutant spores. Although mostsyb1-S1mutant spores are germinated, they are less tolerant to ethanol than wild-type spores. Thesyb1-S1allele carries a missense mutation, resulting in replacement of a conserved cysteine residue adjacent to the transmembrane domain, which reduces the stability and abundance of the Syb1 protein. Taken together, these results indicate that Syb1 plays an important role in both FSM assembly and spore wall formation.