Optical Control of Peroxisomal Trafficking.
Optical Control of Peroxisomal Trafficking.
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DOI：
10.1021/acssynbio.5b00144
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发表时间：
2016-07-15
影响因子：
4.7
通讯作者：
Tucker CL
中科院分区：
文献类型：
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作者：
Spiltoir JI;Strickland D;Glotzer M;Tucker CL
Avena sativa phototropin 1（AsLOV 2）的蓝光响应LOV 2结构域已被用于调节多种蛋白质靶标的活性和与光的结合。在这里，我们使用AsLOV 2来光笼化过氧化物酶体靶向序列，允许过氧化物酶体蛋白输入的光调节。我们产生了一个蛋白质标签，LOV-PTS 1，它可以附加到感兴趣的蛋白质上，利用光将它们导入过氧化物酶体。该方法提供了一种在用户定义的时间在特定细胞中诱导触发过氧化物酶体蛋白运输的方法。
The blue-light-responsive LOV2 domain of Avena sativa phototropin1 (AsLOV2) has been used to regulate activity and binding of diverse protein targets with light. Here, we used AsLOV2 to photocage a peroxisomal targeting sequence, allowing light regulation of peroxisomal protein import. We generated a protein tag, LOV-PTS1, that can be appended to proteins of interest to direct their import to the peroxisome with light. This method provides a means to inducibly trigger peroxisomal protein trafficking in specific cells at user-defined times.