Important role of loops in the transport activity of the mitochondrial ADP/ATP carrier
Important role of loops in the transport activity of the mitochondrial ADP/ATP carrier
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环在线粒体 ADP/ATP 载体转运活动中的重要作用
DOI：
10.1007/bfb0111057
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
E. Majima
中科院分区：
文献类型：
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作者：
H. Terada;E. Majima
The transport mechanism of the bovine heart mitochondrial ADP/ATP carrier was studied using submitochondrial particles. The modifications of the cysteine residues of the carrier by the SH-reagents eosin-5-maleimide (EMA) and N-ethylmaleimide (NEM), and disulfide bond formation between these cysteine residues catalyzed by copper-o-phenanthroline (Cu(OP)2) under various conditions were studied. In particular, the effects of the transport inhibitors carboxyatractyloside (CATR) and bongkrekic acid (BKA), and fluorescein derivatives were examined. From the results, the topology of the carrier in the membrane, dynamic translocations of the loops of the carrier, and the structure of the primary binding site of the transport substrates ADP and ATP were deduced. The loops are concluded to act as both gates in the transport and binding sites for the substrates. Based on the results, a cooperative swinging-loop model is postulated as the transport mechanism of the ADP/ATP carrier.
E. Majima 等人：“使用 SH 试剂曙红 5-马来酰亚胺和 N-乙基马来酰亚胺表征线粒体 ADP/ATP 载体的半胱氨酸残基”J. Biol。
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