Hydrogen sulfide induces apoptosis in epithelial cells derived from human gingiva
Hydrogen sulfide induces apoptosis in epithelial cells derived from human gingiva
复制标题
DOI：
10.1088/1752-7155/2/1/017007
复制
发表时间：
2008-03-01
影响因子：
3.8
通讯作者：
Ii, H.
中科院分区：
文献类型：
--
作者：
Murata, T.;Yaegaki, K.;Ii, H.
硫化氢（H（2）S）不仅是引起口臭的主要原因之一，而且在生物膜相关性牙周病中对牙周细胞和组织具有毒性作用。牙龈上皮细胞凋亡可能在牙周炎的发生、发展中起重要作用。我们使用人牙龈成纤维细胞（HGF）和来源于人牙龈的角质形成细胞样Ca 9 -22细胞，研究了硫化氢对细胞凋亡诱导的影响。通过将H（2）S加入到含有5%CO（2）的空气中，将细胞与H（2）S（100 ng ml（-1））孵育24、48或72 h，在孵育期间不断向培养环境中提供。流式细胞仪Annexin V染色检测H（2）S诱导的细胞凋亡率。与对照组相比，在Ca 9 -22细胞和HGF中，H2S暴露48 h凋亡细胞的比例显著增加。在空气中的浓度为100 ng ml（-1）H（2）S是可能在龈沟。本研究表明，牙龈上皮细胞和HGF的H（2）S的凋亡可能发生在口腔中，这可能会导致牙周疾病。
Hydrogen sulfide (H(2)S) is not only one of the main causes of halitosis but is also an agent of toxicity against periodontal cells and tissues in biofilm-related periodontal diseases. Also, apoptosis of gingival epithelial cells may play an important role in the onset and progress of periodontitis. We examined the effect of H(2)S on the induction of apoptosis, using human gingival fibroblasts (HGF) and keratinocyte-like Ca9-22 cells derived from human gingiva. The cells were incubated with H(2)S (100 ng ml(-1)) for 24, 48 or 72 h by adding H(2)S to air containing 5% CO(2), supplied constantly to the culture environment during incubation. The incidence of apoptosis caused by H(2)S was determined with Annexin V staining by flow cytometry. The proportion of apoptotic cells was significantly increased by exposure to H2S for 48 h in comparison with the control in both Ca9-22 cells and HGF. A concentration of 100 ng ml(-1) H(2)S in air is possible in the gingival sulcus. This study indicates that apoptosis in gingival epithelial cells and HGF by H(2)S may occur in the oral cavity, which may cause a periodontal condition.