Liquid chromatographic/tandem mass spectrometric method for the quantitation of tranilast in human plasma.
Liquid chromatographic/tandem mass spectrometric method for the quantitation of tranilast in human plasma.
复制标题
液相色谱/串联质谱法定量人血浆中曲尼司特。
DOI：
10.1002/rcm.2741
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
J. Gu
中科院分区：
文献类型：
--
作者：
Jing Lan;Jian Wang;Dan Wu;Yingwu Wang;J. Paul Fawcett;J. Gu
建立了以曲马多为内标物的液相色谱-串联质谱法测定人血浆中曲尼司特浓度的分析方法。样品制备涉及用甲醇沉淀蛋白质。使用甲醇/10 mM乙酸铵（70：30，v/v）作为移动的相，通过反相高效液相色谱法进行分离，运行时间为2.4 min。在Q TRAP系统上进行检测，使用多反应监测。该方法在0.06-20 μ g/mL范围内呈线性，日内和日间精密度（相对标准偏差）分别为2.2-2.6%和2.3- 2.9%。准确度（相对误差）<-2.5%。该方法应用于健康志愿者单次口服80 mg曲尼司特的药代动力学研究。
An analytical method for the determination of tranilast in human plasma using tramadol as the internal standard has been developed based on liquid chromatography/tandem mass spectrometry. Sample preparation involved protein precipitation with methanol. Separation by reversed-phase high-performance liquid chromatography using methanol/10 mM ammonium acetate (70: 30, v/v) as mobile phase was complete in a run time of 2.4 min. Detection on a Q TRAP system used multiple reaction monitoring. The method was linear in the range 0.06-20 microg/mL with intra- and inter-day precisions (as relative standard deviation) of 2.2-2.6% and 2.3-2.9%, respectively. Accuracy (as relative error) was <-2.5%. The method was applied in a pharmacokinetic study in healthy volunteers treated with a single 80 mg oral dose of tranilast.