SUBCELLULAR SEPARATION AND MOLECULAR NATURE OF HUMAN HISTOCOMPATIBILITY ANTIGENS (HL-A)
SUBCELLULAR SEPARATION AND MOLECULAR NATURE OF HUMAN HISTOCOMPATIBILITY ANTIGENS (HL-A)
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DOI：
10.1038/247457a0
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发表时间：
1974-01-01
期刊：
影响因子：
64.8
通讯作者：
CRUMPTON, MJ
中科院分区：
文献类型：
--
作者：
SNARY, D;GOODFELLOW, P;CRUMPTON, MJ
组织相容性抗原已被各种方法溶解，特别是蛋白水解酶2-4、超声波5和用3M KCl6或洗涤剂7-8提取。虽然洗涤剂似乎能溶解完整的抗原，但直到最近，这些制剂被证明很难进一步分离，而其他步骤的产物被证明是可以纯化的，但可能是膜结合抗原的片段。这一解释基于以下论点。首先，3MKCl的增溶作用似乎依赖于细胞汁液的存在，并被蛋白酶抑制剂9显著降低。其次，与洗涤剂溶解的抗原10不同，其他程序产生的非聚集产物通常具有相似的分子量范围35,000-55,00010-12。第三，通过木瓜酶消化获得的小鼠和人类组织相容抗原似乎是糖蛋白(3-8%碳水化合物)，而从豚鼠和人类组织通过超声波和3MKCl提取可溶解的抗原被报道为非糖基化的(低于1%的碳水化合物)12。如果后一种程序引起了降解并随后将碳水化合物从带有抗原决定簇的分子中分离出来，这种二分法是可以解释的。
HISTOCOMPATIBILITY antigens have been solubilised by various means1, especially proteolytic digestion2–4, sonication5and extraction with 3 M KCl6or detergents7–8. Although detergents seem to solubilise the intact antigens these preparations have, until recently, proved difficult to fractionate further, whereas the products of the other procedures have proved amenable to purification but probably represent fragments of the membrane-bound antigen. This interpretation is based on the following arguments. First, solubilisation with 3 M KCl seems to depend on the presence of cell sap and was markedly reduced by protease inhibitors9. Second, in contrast to the detergent-solubilised antigen10, the other procedures yield non-aggregated products which, in general, possess similar molecular weights within the range 35,000–55,00010–12. Third, mouse and human histocompatibility antigens obtained by papain digestion seem to be glycoproteins (3–8% carbohydrate), whereas the antigens solubilised from guinea pig and human tissues by sonication and 3 M KCl extraction have been reported to be non-glycosylated (less than 1 % carbohydrate)12. This dichotomy would be explicable if the latter procedures had caused degradation and the consequent separation of the carbohydrate from the molecule bearing the antigenic determinant.