Presence of indicator bacteria, Salmonella and diarrheagenic Escherichia coli pathotypes on mung bean sprouts from public markets in Pachuca, Mexico
Presence of indicator bacteria, Salmonella and diarrheagenic Escherichia coli pathotypes on mung bean sprouts from public markets in Pachuca, Mexico
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DOI：
10.1016/j.foodcont.2012.10.010
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发表时间：
2013-06-01
期刊：
影响因子：
6
通讯作者：
Castro-Rosas, Javier
中科院分区：
文献类型：
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作者：
Cerna-Cortes, Jorge F.;Gomez-Aldapa, Carlos A.;Castro-Rosas, Javier
对绿豆芽中的大肠菌群(CB)、粪大肠菌群(FC)、大肠埃希氏菌、致泻性大肠杆菌(DEP)和沙门氏菌进行了检测。从墨西哥伊达尔戈州帕丘卡的市场上收集了100个芽苗样本。在这些样本中，CB阳性率为100%，FC阳性率为98%，大肠杆菌阳性率为95%，DEP阳性率为10%，沙门氏菌阳性率为5%。已鉴定的DEP包括产肠毒素大肠埃希菌(ETEC)、肠侵袭性大肠埃希菌(EIEC)和产志贺氏菌毒素(STEC)。从2%的样品中分离出ETEC和EIEC，从6%的样品中分离出STEC。在所有STEC阳性样本中均未检测到O157：H7大肠杆菌。Fc与大肠杆菌呈正相关，与DEP呈正相关。CB与DEP、CB与沙门氏菌呈负相关。芽苗样品中沙门氏菌的存在与FC和E.Coli都不相关。这是墨西哥首次从豆芽中分离到ETEC、EIEC和STEC，也是墨西哥首次从绿豆中分离到沙门氏菌。绿豆芽很可能是导致ETEC、EIEC和STEC以及沙门氏菌相关性胃肠炎在墨西哥流行的重要因素。(C)2012爱思唯尔有限公司。保留所有权利。
Coliform bacteria (CB), fecal coliforms (FC), Escherichia coli, diarrheagenic E. coli pathotypes (DEP) and Salmonella frequencies were determined for mung bean (Vigna radiata) sprouts. One hundred sprout samples were collected from markets in Pachuca, Hidalgo state, Mexico. Of these samples, 100% were positive for CB, 98% for FC, 95% for E. coli, 10% for DEP and 5% for Salmonella. Identified DEP included enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC). The ETEC and EIEC were each isolated from 2% of samples, and the STEC from 6% of samples. No E. coli O157:H7 were detected in any STEC-positive samples. Positive correlations were observed between FC and E. coli and between E. coli and DEP. A negative correlation occurred between CB and DEP, and between CB and Salmonella. Neither FC nor E. coli correlated with Salmonella presence in the sprout samples. This is the first report of ETEC, EIEC and STEC isolated from sprouts in Mexico and the first report of Salmonella isolation from mung bean in Mexico. Mung bean sprouts are very probably an important factor contributing to the endemicity of ETEC, EIEC and STEC and Salmonella-related gastroenteritis in Mexico. (c) 2012 Elsevier Ltd. All rights reserved.