patched overexpression causes loss of wingless expression in Drosophila embryos.
patched overexpression causes loss of wingless expression in Drosophila embryos.
复制标题
修补过表达会导致果蝇胚胎中无翅表达的丧失。
DOI：
10.1006/dbio.1994.1200
复制
发表时间：
1994
影响因子：
2.7
通讯作者：
Scott,MP
中科院分区：
文献类型：
--
作者：
Schuske,K;Hooper,JE;Scott,MP
The patched(ptc) segment polarity gone ofDrosophilaencodes a transmembrane protein involved in cell signaling that establishes pattern within the segment. In the posterior half of the parasegment Patched protein represses transcription of thewingless(wg) gene by an unknown mechanism. In the most posterior row of cells in each parasegment this repression is neutralized by a signal possibly carried by the product of thehedgehoggene, allowingwgexpression. High levels of Patched expression might therefore overcome the repression and represswgin all cells. Here we use a heat shock-inducible promoter to transiently express high levels of Patched in all cells. A single pulse of Patched transgene expression has little or no effect on the segmental pattern, as has been previously reported. Repeated pulses of Patched production drastically alter the segment pattern to mimic embryos lacking one of thewgclass of segment polarity genes. We observe repression ofwgandgooseberry(awgclass gene) transcription in the germband ectoderm but not in the head. Expression of two other segment polarity genes,engrailedandcubitus interruptus, is unaffected. Thus excess Patched is capable of overcoming the neutralizing signal.
