On-chip determination of dopamine exocytosis using mercaptopropionic acid modified microelectrodes
On-chip determination of dopamine exocytosis using mercaptopropionic acid modified microelectrodes
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DOI：
10.1002/elan.200603720
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发表时间：
2007-01-01
期刊：
影响因子：
3
通讯作者：
Ruzgas, Tautgirdas
中科院分区：
文献类型：
--
作者：
Spegel, Christer;Heiskanen, Arto;Ruzgas, Tautgirdas
通常用于薄膜金属化的金和铂不适合测量多巴胺（DA），因为DA的氧化产物在电极表面形成不导电的聚合物。在这项工作中，筛选了几种硫醇阻止这种聚合的能力。研究发现，巯基丙酸 (MPA) 会降低 DA 聚合速率。 MPA 具有弱酸性功能，通过减少电极钝化以及提高可逆性和灵敏度，对 DA 电化学产生最大影响。用 MPA 对微芯片电极进行修饰不仅改善了 DA 电化学，还显着提高了传感器的存储稳定性。微芯片最终用于检测 PC12 细胞中 K+ 刺激的 DA 量子释放。
Gold and platinum, which often are used for thin film metallization, are not suitable for the measurement of dopamine (DA), since the oxidation product of DA forms a non-conducting polymer on the electrode surface. In this work several thiols were screened for their ability to prevent this polymerization. It was found that mercaptopropionic acid (MPA) decreased the rate of DA polymerization. MPA, possessing a weak acidic functionality, had the greatest effect on the DA electrochemistry by decreasing electrode passivation, as well as improving reversibility and sensitivity. Modifications of microchip electrodes with MPA did not only improve DA electrochemistry but also significantly increased the storage stability of the transducers. The microchips were ultimately used to detect K+ stimulated quantal release of DA from PC12 cells.