Smartphone confocal microscopy for imaging cellular structures in human skin in vivo
Smartphone confocal microscopy for imaging cellular structures in human skin in vivo
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DOI：
10.1364/boe.9.001906
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发表时间：
2018-04-01
影响因子：
3.4
通讯作者：
Kang, Dongkyun
中科院分区：
文献类型：
--
作者：
Freeman, Esther E.;Semeere, Aggrey;Kang, Dongkyun
我们报告了一种低成本智能手机共聚焦显微镜的开发及其在体内人体皮肤成像的首次演示。智能手机共焦显微镜使用狭缝孔径和衍射光栅进行二维共焦成像，无需使用任何光束扫描设备。智能手机共聚焦显微镜的横向和轴向分辨率分别为2和5 μ m。人体皮肤的活体共聚焦图像显示了特征性的细胞结构，包括棘状和基底角质形成细胞以及乳头状真皮。结果表明，智能手机共聚焦显微镜具有在体内检查细胞细节的潜力，并可能有助于在资源匮乏的环境中进行疾病诊断，其中进行标准组织病理学分析具有挑战性。(c)根据OSA开放获取出版协议的条款，2018年美国光学学会
We report development of a low-cost smartphone confocal microscope and its first demonstration of in vivo human skin imaging. The smartphone confocal microscope uses a slit aperture and diffraction grating to conduct two-dimensional confocal imaging without using any beam scanning devices. Lateral and axial resolutions of the smartphone confocal microscope were measured as 2 and 5 mu m, respectively. In vivo confocal images of human skin revealed characteristic cellular structures, including spinous and basal keratinocytes and papillary dermis. Results suggest that the smartphone confocal microscope has a potential to examine cellular details in vivo and may help disease diagnosis in resource-poor settings, where conducting standard histopathologic analysis is challenging. (c) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement