Protein synthesis and the expression of growth-related genes are altered by running in human vastus lateralis and soleus muscles
Protein synthesis and the expression of growth-related genes are altered by running in human vastus lateralis and soleus muscles
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DOI：
10.1152/ajpregu.90906.2008
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发表时间：
2009-03-01
影响因子：
2.8
通讯作者：
Trappe, Scott W.
中科院分区：
文献类型：
--
作者：
Harber, Matthew P.;Crane, Justin D.;Trappe, Scott W.
Harber MP，起重机JD，Dickinson JM，Jemiolo B，Raue U，Trappe TA，Trappe SW.蛋白质合成和生长相关基因的表达通过在人股外侧肌和比目鱼肌中跑步而改变。Am J Physiol Regul Integr Comp Physiol 296：R708-R714，2009。首次发表于2008年12月31日; doi：10.1152/ajpregu.90906.2008。最近的证据表明，有氧运动可能有助于保持比目鱼肌质量在卸载。这项研究的目的是检查肌肉特定的代谢反应运行，因为它涉及到肌肉生长。在以77 +/- 1%的强度进行45分钟的水平分级跑台跑步之前和之后，对8名男性（26 +/- 2岁; V（过点）O-2 max 63 +/- 2 ml.kg（-1）.min（-1））的股外侧肌（VL）和比目鱼肌（SOL）的混合肌肉蛋白质合成[合成率分数（FSR）]和基因表达（GE）进行了检测。肌糖原利用率在肌肉之间是相似的。VL（0.080 +/-0.007%/h）和SOL（0.086 +/-0.008%/h）之间的静息FSR相似，运动后24 h，两块肌肉的FSR均高于（P < 0.05）静息FSR。肌肉之间FSR的绝对变化无差异（VL和SOL分别为0.030 +/- 0.007和0.037 +/-0.012%/h）。在基线时，SOL中的肌生长抑制素GE大约是VL的两倍（P < 0.05），而GE中不存在其他肌肉特异性差异。运动后4 h左右两块肌肉的肌生长抑制素GE均受到抑制（P < 0.05），24 h仅VL肌的GE高于基线水平（P < 0.05）。肌肉调节因子4 mRNA升高SOL和VL在4 h时均显著高于对照组（P < 0.05）; MyoD和过氧化物酶体增殖物激活受体-γ共激活因子-1 α（PGC-1 α）高于（P < 0.05），而叉头盒[FOXO]3A仅在SOL中在24 h时较高，而肌肉环指蛋白1（MuRF-1）在4 h时仅在VL组高于对照组（P < 0.05）。肌细胞生成素和atrogin-1 GE无变化。FSR中肌肉之间的类似增加支持跑步作为运动对策的一部分，以在卸载期间保持比目鱼肌质量。GE的细微差异表明肌肉特异性适应慢性跑步训练的潜在机制。
Harber MP, Crane JD, Dickinson JM, Jemiolo B, Raue U, Trappe TA, Trappe SW. Protein synthesis and the expression of growth-related genes are altered by running in human vastus lateralis and soleus muscles. Am J Physiol Regul Integr Comp Physiol 296: R708-R714, 2009. First published December 31, 2008; doi:10.1152/ajpregu.90906.2008.-Recent evidence suggests aerobic exercise may help preserve soleus muscle mass during unloading. The purpose of this investigation was to examine the muscle-specific metabolic response to running as it relates to muscle growth. Mixed-muscle protein synthesis [fractional synthetic rate (FSR)] and gene expression (GE) were examined in the vastus lateralis (VL) and soleus (SOL) muscles from eight men (26 +/- 2 yr; V(over dot)O-2max 63 +/- 2 ml.kg(-1).min(-1)) before and after a 45-min level-grade treadmill run at 77 +/- 1% intensity. Muscle glycogen utilization was similar between muscles. Resting FSR was similar between the VL (0.080 +/- 0.007 %/h) and SOL (0.086 +/- 0.008 %/h) and was higher (P < 0.05) 24 h postexercise compared with rest for both muscles. The absolute change in FSR was not different between muscles (0.030 +/- 0.007 vs. 0.037 +/- 0.012 %/h for VL and SOL). At baseline, myostatin GE was approximately twofold higher (P < 0.05) in SOL compared with VL, while no other muscle-specific differences in GE were present. After running, myostatin GE was suppressed (P < 0.05) in both muscles at 4 h and was higher (P < 0.05) than baseline at 24 h for VL only. Muscle regulatory factor 4 mRNA was elevated (P < 0.05) at 4 h in both SOL and VL; MyoD and peroxisome-proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1 alpha) were higher (P < 0.05) at 4 h, and forkhead box [FOXO]3A was higher at 24 h in SOL only, while muscle-RING-finger protein-1 (MuRF-1) was higher (P < 0.05) at 4 h in VL only. Myogenin and atrogin-1 GE were unaltered. The similar increases between muscles in FSR support running as part of the exercise countermeasure to preserve soleus mass during unloading. The subtle differences in GE suggest a potential mechanism for muscle-specific adaptations to chronic run training.