Determination of melamine in powdered milk by molecularly imprinted stir bar sorptive extraction coupled with HPLC.
Determination of melamine in powdered milk by molecularly imprinted stir bar sorptive extraction coupled with HPLC.
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DOI：
10.1016/j.jcis.2015.05.008
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发表时间：
2015-09
影响因子：
9.9
通讯作者：
Ling Zhu;Guanhong Xu;Fangdi Wei;Jing Yang;Qin Hu
中科院分区：
文献类型：
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作者：
Ling Zhu;Guanhong Xu;Fangdi Wei;Jing Yang;Qin Hu
以三聚氰胺（MA）为模板分子制备了一种新型分子印迹搅拌棒（MIP-SB）。MIP-SB的吸附量是非印迹搅拌棒（NIP-SB）的近3倍。MIP-SB对MA及其类似物的选择性明显优于NIP-SB。建立了MIP-SB吸附萃取-高效液相色谱法测定奶粉中MA的方法。线性范围为0.0631 ~ 12.6 ng/mL，相关系数为0.9983，信噪比为3倍，检出限为0.0127 ng/mL。该方法可用于奶粉中MA的测定，结果令人满意。所建立的方法简便，适用于乳制品中MA的测定，对乳制品的质量控制具有重要意义。
A novel molecularly imprinted stir bar (MIP-SB) was developed with melamine (MA) as the template molecule in this study. The sorptive capacity of MIP-SB was nearly three times over that of non-imprinted stir bar (NIP-SB). The MIP-SB presented much better selectivity than NIP-SB when used to absorb MA and its analogues. An analytical method to determine MA in powdered milk was established by combining MIP-SB sorptive extraction with HPLC. The liner range was 0.0631–12.6 ng/mL with good correlation coefficient of 0.9983, and the limit of detection was 0.0127 ng/mL based on three times ratio of signal to noise. This method was successfully applied to the determination of MA in powdered milk with satisfactory results. The method built is simple and suitable for the determination of MA in milk products which is of great significance for quality control of milk products.