High resolution laser mass spectrometry bioimaging.
High resolution laser mass spectrometry bioimaging.
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DOI：
10.1016/j.ymeth.2016.03.002
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发表时间：
2016-07-15
期刊：
影响因子：
--
通讯作者：
Ghorai S
中科院分区：
文献类型：
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作者：
Murray KK;Seneviratne CA;Ghorai S
MSI(MSI)是五十多年前随着二次离子质谱仪(SIMS)而引入的，十年后又随着激光解吸/电离(LDI)质谱学(MS)而引入。基质辅助激光解吸电离(MALDI)生物大分子成像技术(MALDI)是20世纪90年代发展起来的，而环境激光质谱仪是在10年前发展起来的。尽管SIMS从一开始就能够以亚微米的横向分辨率进行中等质量范围的成像，但激光MS需要额外的努力才能达到10μm或更低的横向分辨率，这是在单个哺乳动物细胞大小的尺度上进行成像所必需的。这篇综述包括使用激光解吸/电离或激光解吸和后电离的非靶向生物大分子微卫星。这些方法包括激光微探针(LDI)MSI、MALDI MSI、激光环境和大气压力MSI以及近场激光烧蚀MS。讨论了提高横向分辨率的新方法，包括过采样、光束整形、传输几何、反射和穿透孔物镜、显微镜模式和近场光学。
MSI (MSI) was introduced more than five decades ago with secondary ion mass spectrometry (SIMS) and a decade later with laser desorption/ionization (LDI) mass spectrometry (MS). Large biomolecule imaging by matrix-assisted laser desorption/ionization (MALDI) was developed in the 1990s and ambient laser MS a decade ago. Although SIMS has been capable of imaging with a moderate mass range at sub-micrometer lateral resolution from its inception, laser MS requires additional effort to achieve a lateral resolution of 10 μm or below which is required to image at the size scale of single mammalian cells. This review covers untargeted large biomolecule MSI using lasers for desorption/ionization or laser desorption and post-ionization. These methods include laser microprobe (LDI) MSI, MALDI MSI, laser ambient and atmospheric pressure MSI, and near-field laser ablation MS. Novel approaches to improving lateral resolution are discussed, including oversampling, beam shaping, transmission geometry, reflective and through-hole objectives, microscope mode, and near-field optics.