One-pot quaternization of dual-responsive poly(vinyl alcohol) with AIEgens for pH-switchable imaging and killing of bacteria
One-pot quaternization of dual-responsive poly(vinyl alcohol) with AIEgens for pH-switchable imaging and killing of bacteria
复制标题
使用 AIEgens 对双响应聚（乙烯醇）进行一锅季铵化，用于 pH 切换成像和杀菌
DOI：
10.1039/d0qm00014k
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发表时间：
2020
影响因子：
7
通讯作者：
Ren Li
中科院分区：
文献类型：
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作者：
Wang Jin;Gao Meng;Cui Zhong-Kai;Jia Yong-Guang;Liu Sa;Chen Kai-Feng;Chen Xiaohui;Zhang Yiqing;Fang Zhou;Chen Yunhua;Wang Kaojin;Zhang Huatang;Wang Lin;Ren Li
面对细菌感染带来的挑战，迫切需要结合抗菌功能对细菌感染进行实时检测。在这里，我们用叔胺一锅法简单地制备了温度和pH响应性的聚乙烯醇衍生物(PVA-TPE)，它们含有四苯基乙烯(TPE)和季铵盐基团的聚集诱导发射发光物质。热塑性弹性体的本征荧光和季铵盐单元的抗菌功能可以有效地整合“监测和杀菌”的功能。研究发现，PVA-TPE在pH为7.4时可选择性成像并杀灭革兰氏阳性菌，而在酸性环境(pH 5.5)中对革兰氏阴性菌和革兰氏阳性菌均显示出增强的广谱抗菌活性。PVA-TPE对哺乳动物细胞L929、HepG2和红细胞具有较低的细胞毒性。此外，体内评价进一步证实PVA-TPE具有良好的抗菌效果，且不会对组织造成损害。这项研究是通过简单的一锅季铵化法构建的pH可调的集成系统的第一个例子，用于“监测和杀灭细菌”。
Facing the challenges posed by bacterial infection, the real-time detection of bacterial infection combined with antibacterial functions is urgently needed. Herein, we prepare thermo- and pH-responsive poly(vinyl alcohol) derivatives (PVA–TPE) bearing aggregation-induced emission luminogens of tetraphenylethene (TPE) and quaternary ammonium moieties through a facile one-pot quaternization with a tertiary amine method. Intrinsic fluorescence from TPE moieties and antibacterial functions from quaternary ammonium units can efficiently integrate the functions of “monitoring and killing of bacteria”. It is found that PVA–TPE can selectively image and kill Gram-positive bacteria at pH 7.4, while it switched to display enhanced broad-spectrum antimicrobial activity against both Gram-negative and -positive bacteria in an acidic environment (pH 5.5). Moreover, PVA–TPE exhibits low cytotoxicity on mammalian cells of L929, HepG2 and red blood cells. Furthermore, in vivo evaluations further confirmed that PVA–TPE has excellent antibacterial efficacy with no tissue damage. This study represents the first example of a pH-switchable integrated system for “monitoring and killing of bacteria” constructed through a facile one-pot quaternization method.