Ofloxacin as a reference marker in hair of various colors.
Ofloxacin as a reference marker in hair of various colors.
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氧氟沙星作为各种颜色头发的参考标记。
DOI：
10.1093/jat/27.3.149
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发表时间：
2003
影响因子：
2.5
通讯作者：
Rollins,DouglasE
中科院分区：
文献类型：
--
作者：
Wilkins,DianaG;Mizuno,Atsuhiro;Borges,ChadR;Slawson,MatthewH;Rollins,DouglasE
有人提出，向人类受试者施用可靠的标记物质可以增强药物治疗计划中识别药物使用和治疗依从性的能力。本研究的目的是确定口服抗生素氧氟沙星 (OFLX) 是否可以用作“标记”物质，以建立各种颜色头发的时间参考点。 18 至 40 岁之间的男性和女性受试者 (n = 32) 每天分次口服 800 mg OFLX。受试者被限制剪发或进行化学治疗。在给药前以及给药后第4、5、6和7周收集（通过切割）头发。受试者被分类为黑色 (n= 5)、棕色 (n= 13)、金色 (n= 8) 或红色 (n= 6) 头发。将头发分割成 3.0 厘米的片段，然后进行消化、提取和高压液相色谱 (HPLC) 分析。第 7 周时，最靠近头皮的前 3.0 厘米头发中的平均 OLFX 浓度 (± 1 SD) 如下：30.6 ± 8.5 ng/mg（黑色）、6.0 ± 1.8 ng/mg（棕色）、3.5 ± 1.6 ng/mg（金发）和 1.4 ± 0.3 ng/mg（红色）。在第 4-6 周收集的头发中也发现了类似的模式。还通过 HPLC 测定了每个受试者的定量真黑素 (EUM) 毛发浓度。 7 周时 OFLX 浓度与 EUM 之间存在密切关系（r2 调整 = 0.728；p< 0.001）。在六名受试者中，我们还确定了 OFLX 掺入个别发束的受试者内变异性。来自每个受试者的四根链被分割成 2 毫米的片段并进行分析。 OFLX 在第 5 周出现在 #1–#10 段（头发的第一厘米）。 OFLX 在第 7 周出现在#2–#20 段（头发的第一厘米和第二厘米）。然后确定每条链的最大 OFLX 浓度（药物“带”）和位置。在第 5 周时，测量所有受试者的#2-#5 段的最大 OFLX 浓度（头发长度的第一厘米内）。第 7 周时，在 #3#2–#5#8 段（头发第一厘米和第二厘米内）测量了最大 OFLX 浓度。尽管发现了相当大的个体间变异性，但这与小于 1.0 厘米/月的生长速率是一致的。没有注意到 OFLX 沿着毛干超出头发的前 3.0 厘米的显着轴向扩散。尽管头发颜色有很强的影响，但这些数据表明 OFLX 可能是一种合适的头发标记物质，允许受试者作为自己的“对照”。未来的研究将探索是否可以借助 OFLX 来确定临床药物滥用研究中的药物使用、治疗依从性或累犯情况。
It has been proposed that administration of a reliable marker substance to human subjects may enhance the ability to identify drug use and treatment compliance in drug treatment programs. The goal of this study was to determine if an oral dose of the antibiotic ofloxacin (OFLX) could be used as a “marker” substance to establish reference points with respect to time in hair of various colors. Male and female subjects (n= 32) between 18 and 40 years of age received 800 mg of OFLX as a divided oral dose on a single day. Subjects were restricted from cutting their hair or performing chemical treatments. Hair was collected (by cutting) before, and at weeks 4, 5, 6, and 7 after drug administration. Subjects were classified as having black (n= 5), brown (n= 13), blonde (n= 8), or red (n= 6) hair. Hair was segmented into 3.0-cm segments prior to digestion, extraction, and analysis by high-pressure liquid chromatography (HPLC). At 7 weeks, the mean OLFX concentrations (± 1 SD) in the first 3.0 cm of hair closest to the scalp were as follows: 30.6 ± 8.5 ng/mg (black), 6.0 ± 1.8 ng/mg (brown), 3.5 ± 1.6 ng/mg (blonde), and 1.4 ± 0.3 ng/mg (red). A similar pattern was found in hair collected at weeks 4–6. Quantitative eumelanin (EUM) hair concentrations for each subject were also determined for each subject via HPLC. A strong relationship between OFLX concentration at 7 weeks and EUM was noted (r2adjusted = 0.728;p< 0.001). In six subjects, we also determined the intrasubject variability of OFLX incorporation into individual hair strands. Four strands from each subject were segmented into 2-mm segments and analyzed. OFLX appeared in segments #1–#10 at week 5 (the first centimeter of hair). OFLX appeared in segments #2–#20 at week 7 (the first and second centimeter of hair). The maximum OFLX concentration (the “band” of drug) and location was then determined for each strand. The maximum OFLX concentration was measured in segments #2–#5 at week 5 for all subjects (within the first centimeter of hair length). The maximum OFLX concentration was measured in segments #3#2–#5#8 at week 7 (within the first and second centimeter of hair). This was consistent with a growth rate of less than 1.0 cm/month, although considerable intersuhject variability was found. No significant axial diffusion of OFLX along the hair shaft beyond the first 3.0 cm of hair was noted. Despite a strong effect of hair color, these data suggest that OFLX may be a suitable marker substance for hair, allowing a subject to serve as their own “control” Future studies will explore whether drug use, treatment compliance, or recidivism in clinical drug-abuse studies can be determined with the aid of OFLX.